IHC二抗放大检测系统质控片的制备及评价  

Preparation and evaluation of control slides used for detecting secondary antibody amplification system in immunohistochemistry

作　　者：林齐心[1] 王小亚[2] 黄以鹏[1] 郑美云[1] 孟春[1] 

机构地区：[1]福州大学迈新生物肿瘤诊断研究所,福州350108 [2]福州迈新生物技术开发有限公司,福州350002

出　　处：《诊断病理学杂志》2013年第8期498-501,共4页Chinese Journal of Diagnostic Pathology

摘　　要：目的本研究分别采用不同表面修饰方法处理的玻璃载玻片为基质,以一抗为对象制备二抗放大检测系统质控片。方法将小鼠IgG作为探针固定在玻片上,采用二抗放大检测系统对不同质控片的吸附结合效果进行评价。结果戊二醛预处理的载玻片和硅烷化预处理的载玻片对蛋白吸附较好,反应活性高。结论通过不同二抗放大检测系统的比较表明,MaxVisionTM和EliVisionTMSuper放大检测系统的检测灵敏度高,检测范围宽。EliVisionTMSuper放大检测系统的显示灰度值与检测对象浓度的对数值线性化程度最高。Objective To prepare the control slides for immunohistochemistry quality control of secondary antibodies by combined primary antibodies on glass slides modified with different surface modificated ways.Methods The combined efficiency of mouse IgG on different slides was evaluated with a secondary antibody amplification system.Results The results showed that both the aldehyde modified slides and silylated slides exhibited high adsorption efficiency to proteins and satisfied reactive activities.Conclusion The evaluated results of different commercial secondary antibody amplification systems with silylated slides show that MaxVisionTM and EliVisionTM super have a large detection limit and high sensitivity.EliVisionTM super amplification system has the highest linear correlation between the logarithmic IgG concentrations and absorption values.

关 键 词：二抗放大检测系统 免疫组化 质量控制 

分 类 号：R334[医药卫生—人体生理学]