高效表达乙型肝炎病毒表面抗原的CHO细胞株的建立  

Construction of recombinant CHO cell strain for high expression of HBsAg

作　　者：伊瑶[1] 郭敏卓[2] 田瑞光[1] 苏秋东[1] 卢学新[1] 邱丰[1] 周文亭[1] 贾志远[1] 毕胜利[1] 

机构地区：[1]中国疾病预防控制中心病毒病研究控制所,北京102206 [2]北京出入境检验检疫局保健中心

出　　处：《中华实验和临床病毒学杂志》2013年第4期289-291,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探索乙型肝炎病毒表面抗原（s）基因在CHO无血清细胞培养系统中的高效表达。方法构建包含乙肝病毒s基因的质粒，转染CHO细胞，经MTX筛选后对培养液上清中的表达产物进行血清学检测以及形态学的观察。结果获得了能高效稳定表达HBsAg的CHO细胞株，电镜下可观测到小球型和管型颗粒，ELISA检测表达产物滴度达到1：5000。结论成功构建高效表达乙型肝炎病毒s蛋白的CHO无血清培养细胞株，表达的HBsAg具有良好的抗原活性。Objective To overexpress hepatitis B virus S gene in CHO cells cultured in serum-free media. Method Plasmid was constructed by cloning of HBV S gene and then it was transfected into CHO cells. After cell screen, the positive clones were identified and isolated into a serum-free media followed by the serological and morphological characterization of the expression product. Result CHO cell strains which can express HBsAg efficiently and stably were obtained. Spherical and filamentous HBsAg could be detected under electronic microscope. The titer of the expression product was up to 1 ： 5000. Conclusion Serum-free media cultured CHO cell strain for overexpression of HBsAg was successfully constructed and the expression product was high antigenic.

关 键 词：肝炎病毒 乙型 肝炎表面抗原 乙型 细胞培养 

分 类 号：R51[医药卫生—内科学]