L-选择素基因型及血清水平与系统性红斑狼疮的相关性  

Association of the Genotype and Serum Level of L-selectin with Systematic Lupus Erythematosus

作　　者：蓝艳[1] 蒋远文[1] 罗斌[2] 李北林[2] 韦彩成[2] 

机构地区：[1]广西百色市右江民族医学院附属医院皮肤科,广西百色533000 [2]广西百色市右江民族医学院附属医院检验科,广西百色533000

出　　处：《中国皮肤性病学杂志》2013年第9期881-883,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(81260234);广西卫生厅重点基金资助课题(Z2009269)

摘　　要：目的探讨L-选择素基因单核苷酸多态性与系统性红斑狼疮(SLE)易感性之间的关系;同时分析L-选择素基因型及血清水平与SLE的相关性。方法以145例SLE患者和160例健康对照者为研究对象,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和DNA测序的方法对L-选择素基因P213S单核苷酸多态性进行基因分型,同时采用酶联免疫吸附试验检测血清L-选择素水平。结果在SLE组中,L-选择素三种基因型PP,PS,SS分布频率分别为54.48%,38.62%和6.90%;在对照组中其分别为51.88%,40.00%和8.13%。L-选择素基因P213S多态性在SLE组和正常人群中的分布频率差异无统计学意义(P>0.05),但携带PP基因型的SLE患者血清L-选择素水平显著高于不携带者,差异有统计学意义(P<0.05)。SLE组血清L-选择素水平明显高于对照组,差异有统计学意义(P<0.05)。结论 L-选择素基因P213S多态性与SLE的易感性无关,但PP基因型携带者可能与SLE的发病风险相关。Objective To investigate of the single nucleotide polymorphism of L-selectin gene are associated with susceptibility of systematic lupus erythematosus （SLE） in a Chinese population, and to analyze the association of serum levels and genotypes of L-selectin with SLE. Methods We analyzed L-selectin gene P213S single nucleotide polymorphism in 145 patients with SLE and 160 age and sex matched controls in a Chinese population,using a polymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP） strategy and DNA sequencing, and the serum levels of L-selectin was determined by enzyme-linked immunosorbent assay （ELISA）. Results The frequencies of PP,PS and SS genotype were 54.48% ,38.62% and 6.90% in SLE patients respectively, while in controls were 51.88% ,40.00%, and 8.13%. The distributions of L- selectin gene P213S polymorphism was not significantly different between SLE and control group （P 〉 0.05 ）, the serum levels of L-selectin PP genotype carriers was significantly higher than that of no carriers （ P 〈 0.05）. L-selectin serum levels of the patients with SLE were significantly higher than those of the controls （P 〈 0.05 ）. Conclusion L-selectin gene P213S polymorphism are not associated with susceptibility of SLE, Carrying L-selectin PP genotype increase the risk of SLE.

关 键 词：L-选择素 系统性红斑狼疮 基因 基因型频率 

分 类 号：R593.24[医药卫生—内科学]