Nodal基因5'末端侧翼序列增强子的分析  

Analysis of an Inhancer Element of Mouse Nodal Gene in its 5' Flanking Sequence

作　　者：胡新立[1] 周迅蕾[2] 吴鹤龄[3] 

机构地区：[1]美国密尼苏达大学医学院药学系 [2]德国Max-Plank生物物理化学研究所分子细胞生物学系 [3]北京大学生命科学学院,北京100871

出　　处：《Acta Genetica Sinica》2000年第3期202-209,共8页

基　　金：国家自然科学基金资助!39470371 This project was supported by National Naturol Science Foundation of China(Project number

摘　　要：ＮＯｄａｌ基因属于ＴＧＦ－β超家族，它在小鼠胚胎早期发育的中胚层形成过程中起着重要 的作用。Ｎｏｄａｌ基因缺失的小鼠由于没有中胚层的形成导致死亡。分析了Ｎｏｄａｌ基因的侧翼 序列。利用荧光素酶为报告基因，构建了一系列含有侧翼序列亚克隆及其缺失体报告基因质 粒，并转化了Ｆ９细胞。分析这些亚克隆及其缺失体对报告基因活性的影响。在Ｎｏｄａｌ基因上 约－２ｋｂ处有一个增强子元件存在并鉴定了它的细胞种类特异性。Nodal, a member of TGF-β superfamily, plays a very important role in the mesoderm formation during mouse early development. Mice without the Nodal gene will die due to the lack of mesoderm. The flanking sequences of Nodal were dissected. By using lucifirase as reporter gene, F9 cells were transfected with a series of reporter constructs containing the subclones of the flanking sequences and their nested deletions. The effect of these subclones and deletions-2kb up stream the Nodal gene and its cell type spicificity was determined.

关 键 词：NODAL基因 表达调控 顺式元件 

分 类 号：Q786[生物学—分子生物学]