LAS对雄性小鼠及其子代生精功能毒性作用的研究  

Study on the toxicity of LAS to male mice and male offspring spermatogenic function

作　　者：韩海艳[1] 任春娥[2] 乔鹏云[1] 姜俊怡[1] 张茂翔[1] 

机构地区：[1]潍坊医学院附属医院,潍坊261000 [2]潍坊医学院,潍坊261000

出　　处：《现代妇产科进展》2013年第8期622-625,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨十二烷基苯磺酸钠(LAS)对雄性小鼠及其子代雄性小鼠生精功能的毒性作用。方法:将120只昆明种雄性小鼠随机分为4组,每组30只。实验A组:小鼠经灌胃方式染毒LAS(630mg/kg,1/2LD50),每天1次,连续8周后与正常雌鼠交配(交配比例1∶1)。实验B组:小鼠经灌胃方式染毒LAS,每天1次,连续8周,停止染毒4周后与正常雌鼠交配(交配比例1∶1)。两组交配后雄性小鼠即拉颈处死。实验A、B组的对照组分别为a、b组,以生理盐水灌胃后与正常雌鼠交配。4组所生子代雄性小鼠分别为A1、B1,a1、b1,养至6～8周后处死。比较各组小鼠的体重,睾丸重量,精子密度、活力及形态,睾丸的组织学结构和超微结构。结果:与对照组比较,实验组雄性小鼠及其子代雄性小鼠的精子密度、活率显著下降,畸形率显著升高;雄性小鼠精细小管内的各级精母细胞和精子细胞数量减少,生精细胞排列紊乱;精细小管内的间质细胞、支持细胞、精原细胞内线粒体出现不同程度嵴断裂,空泡样改变及髓样变,破坏广泛。停止染毒后4周,上述异常改变未见明显恢复。结论:LAS对雄性小鼠生精功能具有明显的毒性作用,且该毒性作用在停止染毒后的短时期内不能恢复。Objective：To study the toxicities impact of the linear alkylbenzene sulfonates on the male mice and offspring male reproductive function.Methods：All of the 120 Kunming male mice were divided into four groups randomly and every group was 30.Group A was treated P.O.with LAS 630mg/kg,one time per day,for eight weeks and then mated with female mice（the proportion was 1∶1）.Group B was treated P.O.with LAS 630mg/kg,one time per day,for eight weeks and stopped treating for 4 weeks,then mated with female mice（the proportion was 1∶1）.Both of the male mice of the group A and group B were pulled neck to death after mating.Group a and group b were the contrast groups of group A and group B separately.They were treated with physiological saline solution and pulled neck to death after mating.The offspring male mice of the group A,B,a,b was group A1,B1,a1,b1 separately and pulled neck to death when they growed upto 6～8 weeks.The weight of the body and testicle,concentration and vitality of sperm,sperm forms,histophthology and electron transmission microscopy of the testis were assayed.Results：Compared with the controls,lower of sperm concentration and vitality and higher malformation were obvious.The histology revealed that each spermatocytes and spermids obviously reduced,spermatogenic cells arranged chaoticly.Determination revealed that the morphology of mitochondria in spermospore,leydig cells and sertoli cells from the experimental group showed a greater variance than that in mice from the control group.Furthermore,there showed no obvious recovery of all the morphological abnormalities after be stopped treating with LAS for 4 weeks.Conclusion：LAS has evident toxicities impact on the male animals reproductive function,and the damage the LAS has made could not recovery in a short time.

关 键 词：十二烷基苯磺酸钠 毒性 生精细胞 小鼠 生精功能 

分 类 号：R711.6[医药卫生—妇产科学]