抗苗勒管激素及其Ⅱ型受体与卵母细胞成熟的关系  

Relationship between anti-mullerian hormone,anti-mullerian hormone receptor type Ⅱ and oocyte maturation

作　　者：姜宙[1] 王晓燕[2] 孙贤华[2] 邹淑花[2] 

机构地区：[1]青岛大学医学院,青岛266021 [2]青岛市妇女儿童医疗保健中心生殖中心,青岛266011

出　　处：《现代妇产科进展》2013年第8期635-637,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨抗苗勒管激素(AMH)及其Ⅱ型受体(AMHⅡ)与卵母细胞成熟的关系。方法:选取因男性因素不孕进行卵胞浆内单精子注射(ICSI)治疗的32例患者,于取卵日采集3ml静脉血,收集卵泡液及卵丘颗粒细胞,记录获卵数、卵子成熟度。采用ELISA法测定血清及卵泡液中的AMH水平;Real-time PCR测定卵丘颗粒细胞中AMHⅡ型受体(AMHRⅡ)mRNA的相对表达。结果:卵泡液中的AMH水平[(3083.78±682.19)pg/ml]显著高于血清[(1012.29±423.21)pg/ml](P<0.001);卵泡液中AMH水平与血清AMH水平/获卵数呈正相关(P=0.045)。MⅠ期卵母细胞卵泡液中的AMH水平显著高于MⅡ期(P=0.000);MⅠ期卵丘颗粒细胞中的AMHRⅡmRNA的表达量显著高于MⅡ期(P=0.000)。结论:AMH可能通过Ⅱ型受体抑制卵母细胞成熟;血清AMH/获卵数可作为预测卵母细胞成熟度的参考因子。Objective：To investigate the relationship among anti-mullerian hormone（AMH）,anti-mullerian hormone receptor type Ⅱ expression and oocyte maturation.Methods：Cumulus granular cells,follicular fluids and blood samples were obtained from 32 ovulatory women undergoing intracytoplasmic sperm injection treatment on ovum pick-up day.All the samples were recorded oocyte maturation and the number of retrieved oocytes.The influence of serum and follicular fluids AMH basal levels were evaluated by ELISA.Cumulus GC were assayed by Realtime PCR for AMHRⅡ mRNA expression.Results：AMH concentrations in the FF were higher than that of serum and showed significant differences[（3083.78±682.19）pg/ml vs（1012.29±423.21）pg/ml,P＆lt;0.001].AMH concentrations in the FF and serum level of each retrieved oocytes were found positive correlation（P=0.045）.AMH concentrations in the FF of MⅡ oocytes were significantly lower than in the FF of MⅠoocytes（P=0.000）.AMHRⅡmRNA expression in cumulus GC in MⅠoocytes were 4 times more than MⅡ oocytes（P=0.000）.Conclusion：AMH may inhibit the maturation of oocyte via type Ⅱ receptor.Serum AMH level of each retrieved oocytes could be used as a diagnostic criterion of oocyte maturation.

关 键 词：抗苗勒管激素 抗苗勒管激素Ⅱ型受体 卵母细胞成熟度 卵丘颗粒细胞 

分 类 号：R711.6[医药卫生—妇产科学]