巴马小型猪不同发情周期的阴道细胞学观察  

Vaginal Cytologic Examination on Estrous Cycle in Mini-pig

作　　者：田芳[1] 夏敏杰[1] 王玉柱[1] 周新初[2] 李卫华[1] 丁训诚[1] 

机构地区：[1]上海市计划生育科学研究所,上海200032 [2]上海交通大学农业与生物学院,上海201101

出　　处：《实验动物与比较医学》2013年第4期271-274,共4页Laboratory Animal and Comparative Medicine

基　　金：国家科技重大专项（2012ZX10001-007-009）;十二五国家科技支撑项目（2012BA131804）;国家自然科学基金青年基金项目（81100459）;上海市科委项目（11PJ1408400,11140902900）

摘　　要：目的研究广西巴马小型猪不同发情周期的阴道细胞学特点。方法选取发情间期、发情前期和发情期小型猪各4只，阴道涂片，分别进行HE染色和巴氏染色。光学显微镜下观察阴道涂片细胞形态，进行分类和计数。结果广西巴马小型猪阴道涂片中HE染色和巴氏染色均可见白细胞、副基细胞、小中间细胞、大中间细胞和角化上皮细胞，发情间期：白细胞百分比明显高于发情前期和发情期（P〈0．01）；发情前期：副基细胞和小中间细胞明显高于发情间期和发情期（P〈0．01）；发情期：大中间细胞和角化上皮细胞明显高于发情间期和发情前期（P〈0．01）。结论阴道涂片法可作为确定广西巴马小型猪发情周期的辅助方法，对小型猪的繁殖育种有一定的应用价值。Objective To examine the vaginal cytology of estrous cycle in Guangxi Bama mini-pig. Methods Twelve mini-pig were selected and divided into different groups-diestrus group, proestrus group and estrus group. Vaginal smears of the pigs were obtained and stained with hematologic stain and Papanicolaou stain respectively .Epithelial cell morphology was observed and counted under the optical microscope. Results Leukocytes, para basal cell, small intermediate cell, large intermediate cell and superficial cell were found in vaginal smears. Leukocyte percentage was significantly higher during diestrus than that during estrus or proestrus （P〈0.01）. Para basal cell plus small intermediate cell counts were significantly higher during proestrus than that during estrus or diestrus （P〈0.01）. Superficial plus large intermediate cell counts were significantly higher during estrus than that during diestrus or proestrus （P〈0.01）. Conclusion Vaginal smears can be used to estimate different stage of estrous cycle in Guangxi Bama mini-pig.

关 键 词：阴道涂片 发情周期 小型猪 

分 类 号：Q95-33[生物学—动物学]