裸鼹鼠与C57BL／6小鼠自噬调节的比较研究  

Comparative Study on Autophagy Regulation between Naked Mole Rat and C57BL/6 Mouse

作　　者：林丽芳[1] 赵懿宁[2] 赵善民[1] 汤球[1] 孙伟[1] 张璐[1] 袁子彦[1] 徐晨[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433 [2]上海市杨浦区精神卫生中心,上海200090

出　　处：《实验动物与比较医学》2013年第4期301-305,共5页Laboratory Animal and Comparative Medicine

基　　金：上海市科技发展基金编号（11140901700及12140900200）

摘　　要：目的比较裸鼹鼠与小鼠的自噬调节，以期为裸鼹鼠高自噬、抗癌、衰老缓慢等生物学特性的研究提供参考。方法利用电镜技术和蛋白印迹检测新生裸鼹鼠和C57BL／6小鼠肝脏和肺脏组织的自噬水平。自噬干扰裸鼹鼠成纤维细胞和小鼠成纤维细胞，24、48、72hCCK-8细胞计数法检测细胞的增殖情况，蛋白印迹检测各组细胞各个时间点Beclin1的表达水平。结果与C57BL／6小鼠比较，裸鼹鼠组织的LC3B表达显著较高。裸鼹鼠细胞对自噬抑制剂3-MA具有一定的耐药性，自噬诱导剂雷帕霉素对裸鼹鼠细胞的增殖抑制显著高于小鼠细胞。结论裸鼹鼠组织具有高自噬水平，应对外界的自噬抑制压力时，裸鼹鼠细胞的自身调节能力明显高于C57BL／6小鼠。Objective A comparative study between naked mole rat and C57BL/6 mouse on autoph- agy regulation is conducted, providing a reference for the research of mechanism in high autophagy, anti-cancer, slow aging of NMR. Methods Electron microscopy and western blotting are use to detect the activtity of autophagy in the liver and lung tissue of newborn naked mole rat and C57BL/6. Reagents interfere the autophagy level of fibroblasts derived from naked mole rat and mouse. The cell number is tested after 24, 48, 72 hours by CCK-8 kit. Meanwhile, expression of Beclin 1 at each time point is detected by western blotting. Results Compared with mice, the naked mole rat tissue have significantly higher LC3B expression. The NMR cells have a certain amount of resistance from 3-MA. Rapamycin＇s proliferation inhibition on NMR-S（fibroblasts derived from skin of naked mole rats）was significantly greater than that of mouse cells.Conclution NMR tissue possess a high autophagy, Cells from NMR have greater ability of self-regulation under extraneous autophagy inhibition, compared to C57BL/6.

关 键 词：裸鼹鼠 C57BL 6小鼠 自噬调节 比较研究 

分 类 号：Q95-33[生物学—动物学]