同步分子检测两种腥黑粉菌近似种Tilletia bromi和T.fusca  

Simultaneous molecular detection of two allied smut species—Tilletia bromi and T.fusca

作　　者：廖芳[1] 罗加凤[1] 刘跃庭[1] 刘鹏[1] 黄国明[1] 

机构地区：[1]天津出入境检验检疫局,天津300461

出　　处：《菌物学报》2013年第5期838-845,共8页Mycosystema

基　　金：科技部科技支撑项目(No.2012BAK11B02);国家质检总局课题(No.2008IK236和No.2012IK286);天津出入境检验检疫局课题(No.TK028-2009)

摘　　要：早熟禾是优良的牧草和草坪草,近年来,从进境早熟禾上多次截获一种腥黑粉菌,但一直被鉴定为禾草腥黑粉菌Tilletia fusca。通过比较研究,作者已将该菌种名改为雀麦腥黑粉菌T.bromi。依据T.bromi和T.fusca的序列差异位点设计了6对引物,成功建立了适合菌丝检测的T.bromi和T.fusca的双重PCR方法和适合冬孢子检测的套式双重PCR方法,检测灵敏度达到10pg/μL,为早熟禾腥黑粉菌鉴定提供了快速、可靠的检测方法。Species of Poa are commercially important herbages and turfgrasses in China. In recent years, many samples of bluegrass imported from abroad were found to contain large amount of Tilletia teliospores. The pathogen was often identified as T. fusca. The authors previously considered the species should be T. bromi based on series of comparative morphological investigation. In accordance with to the nucleotide difference of T. bromi and T. fusca, six pairs of primers were designed for double PCR and double nested-PCR amplification. The results showed that T. bromi and T. fusca could be detected and differentiated between each other by applying their mycelial genomic DNA and teliospore genomic DNA. Its sensitivity could reach to 10μg DNA from mycelia or a few teliospores. The methods developed in this study are helpful for quick detection in bluegrass quarantine

关 键 词：早熟禾 雀麦腥黑粉菌 禾草腥黑粉菌 双重检测 套式PCR 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]