大玉竹低聚糖硫酸酯抗HSV-2病毒活性的研究  

STUDY ON ANTIVIRUS ACTIVITY OF SULFATED POLYGONATUM ODORATUM OLIGOSACCHARIDE

作　　者：杨敏[1] 蒙义文[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2000年第5期483-486,共4页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院成都地奥科学基金!(DASF)资助

摘　　要：从传统中药大玉竹中提取低聚糖Poos ,用吡啶 氯磺酸法制备了它的硫酸酯衍生物S Poos.产物经红外鉴定 ,证实Poos硫酸酯化后羟基连上了硫酸基 ,硫含量为 8.47% ,取代度 (Ds)为 0 .6 .体外实验研究了Poos、S Poos对非洲绿猴肾细胞 (Vero)的细胞毒性和抗单纯疱疹病毒 2型 (HSV 2 )的活性 .结果表明 ,Poos和S Poos都无细胞毒性 .Poos本身无抗病毒活性 ,对HSV 2引起的细胞病变和空斑形成都无抑制作用 ;经硫酸酯衍生后的S Poos获得抗病毒活性 ,12 5～ 2 0 0 0 μg/mL的S Poos能很好地抑制HSV 2引起的细胞病变 ,2 0 0 μg/mL的S Poos能完全抑制病毒空斑形成 .图 4表 2参Poos, a kind of oligosaccharide extracted from Polygonatum odoratum (Mill.) Druce (a kind of Chinese herb), was sulfated to obtain S Poos with chlorosulfur acid pyridine. IR showed adsorption at 1 250, 810 cm -1 . The sulfur content of S Poos was found to be 8.47%. The average degree of sub stitution was 0.6. The hydroxy group of S Poos was esterified by chlorosulfur acid. Cytotoxicity in Vero cell, and antivtus activity of Poos and S Poos were investigated in vitro. Poos and S Poos both showed no cytotoxi city in Vero cell. Poos itself did not inhibit the cytopathic effect and plaque forming of Herpes simplex virus type 2(HSV 2) in Vero cell, but its sulfated de rivative obtained antivirus activity, which could well inhibit the cytopathic effect of HS V 2 at concentrations of 125～2 000 μ g/mL, and completely inhibit plaque forming of HSV 2 at concentrations as low as 200 μ g/mL. Fig 4, Tab 2, Ref 12

关 键 词：大玉竹低聚糖 硫酸酯 单纯疱疹病毒 抗病毒活性 

分 类 号：R285[医药卫生—中药学]