两种新蚤细胞色素氧化酶亚基II基因的克隆和测序  

CLONING AND SEQUENCE ANALYSES OF COII GENE OF TWO NEOPSYLLA SPECIES

作　　者：鲁亮[1] 吴厚永[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《寄生虫与医学昆虫学报》2000年第3期170-174,共5页Acta Parasitologica et Medica Entomologica Sinica

基　　金：军事医学科学院创新基金

摘　　要：本文报道了二齿新蚤和宽新蚤线粒体 DN A中一段 90 0碱基的片段 ,其中包括完整的 COII基因和两个氨基酸 t RNA基因片段。两种新蚤 COII基因的 CG含量都是 33.3% ,这在昆虫中是较高的。两者基因间碱基的变异率为 1.8% ,变异以 T→ C转换为主 ,显示两种新蚤间较近的亲缘关系。两种新蚤氨基酸 t RNA基因碱基的变异率低于 0 .5%。两种新蚤的 COII基因和猫栉首蚤 COII基因间碱基的变异率大于 2 0 %。同时列出了完整的细胞色素氧化酶亚基 II氨基酸序列。并列出了在这个基因片段中一些保守序列的位置。A fragment of mtDNA is cloned and sequeenced from Neopsylla bidentatiformis and Neopsylla mana, which includes a complete COII gene and flanking partial Leu tRNA gene and Lys tRNA gene.The CG content of COII gene is 33 3% for two species,which is higher in insect.The sequence difference of COII gene between the two species is 1 8% and is caused mainly by the transitions of T→C.It is suggested that these two species have an intimate relationship.The difference of two tRNA genes is lower than 0 5%.The amino acid sequence difference of COII between the two Neopsylla species is 2%.The sequence difference of COII gene among Neopsylla and Ctenocephalides felis is more than 20%.In addition,some of the conservative sequences in COII gene are labled.

关 键 词：二齿新蚤 宽新蚤 细胞色素化酶亚基Ⅱ基因 

分 类 号：R384[医药卫生—医学寄生虫学]