柳珊瑚共附生放线菌Streptomyces sp.SCSGAA0009中生物碱类化合物及其抗菌和抗附着活性  

Alkaloids from gorgonian-derived Streptomyces sp.SCSGAA0009 and their antibacterial and antilarval activities

作　　者：农旭华[1,2] 张晓勇[1] 陈茵 漆淑华[1] 

机构地区：[1]中国科学院南海海洋研究所广东省海洋药物重点实验室/中国科学院海洋生物资源可持续利用重点实验室,广州510301 [2]中国科学院大学,北京100049 [3]深圳市第九人民医院,深圳518116

出　　处：《微生物学报》2013年第9期995-1000,共6页Acta Microbiologica Sinica

基　　金：国家"973前沿领域项目"(2010CB833800);国家自然科学基金项目(40931160435;40976090)~~

摘　　要：【目的】从南海柳珊瑚共附生放线菌的次生代谢产物中寻找具有抗菌和抗附着活性的先导化合物。【方法】应用化学与生物活性相结合的筛选方法,从柳珊瑚共附生微生物中筛选获得代谢产物丰富且具有生物活性的目标菌株并通过大发酵提取浸膏,利用硅胶柱色谱、凝胶柱色谱和高效液相色谱等方法对发酵产物进行分离、纯化,运用波谱解析鉴定化合物的结构。【结果】从采自海南三亚的柳珊瑚(Muricella flexuosa)样品中分离到一株放线菌SCSGAA0009,鉴定为链霉属Streptomycessp.,从其改良ISP2发酵液中分离到新化合物N-(2-(1H-indol-3-yl)ethyl)propionamide(1)和已知化合物phenazine-1-carboxylic acid(2),其中化合物2对大肠杆菌和海洋细菌假单胞菌(Pseudoaltermonas piscida)具有较好抗菌活性,且有强抗草苔虫(Bugulaneritina)幼虫附着活性。【结论】首次从柳珊瑚共附生放线菌的次生代谢产物中获得新的生物碱化合物1,首次报道化合物2的抗海洋细菌活性和抗附着活性;从南海柳珊瑚共附生微生物的次生代谢产物中可以得到新化合物和活性化合物,这一来源的微生物资源值得深入研究。[ Objective] To search for structurally novel and biologically active compounds from the secondary metabolites of gorgonian-derived actinomyces. [ Methods ] Strains of actinomyces with antimicrobial activities were screened by biological methods. Then, those active strains were cultured under different conditions to obtain crude extracts. Subsequently, the chemical diversities of the extracts were investigated by reverse phase high-performance liquid chromatography （RP-HPLC） and thin layer chromatography （TLC）, while the bioactivities of the extracts were evaluated by antifoulingand antibacterial assays. A target strain Streptomyces sp. SCSGAA0009 was selected to further study by large-scale fermentationon modified ISP2 medium. The compounds were isolated by column chromatography over silica gel, Sephadex LH-20, and semipreparative HPLC, and their structures were determinated by spectroscopic analysis. [ Results] The target strain was identified as actinomyces, Streptomyces sp. SCSGAA0009. A new alkaloid N-（2-（ 1H- indol-3-yl）ethyl） propionamide （1） and a known compound phenazine-l-carboxylic acid （2） were purified from the culture broth. Compound 2 showed moderate antibacterial activity against Escherichia coli and Pseudoaltermonas piscida, and exhibited strong antilarval settlement activity towards Bugula neritina larva. [ Conclusion ] Bioactive and new compounds can be achieved from the secondary metabolites of gorgonian-derived microorganisms from South China Sea.

关 键 词：海洋放线菌Streptomyces sp  生物碱 结构鉴定 抗菌活性 抗附着活性 

分 类 号：R284[医药卫生—中药学]