乳酸杆菌与细菌性阴道病相关致病菌之间的关系  

Association between lactobacillus species and bacterial vaginosis associated bacteria in non-pregnant women

作　　者：陈佳宁[1] 吕志[1] 苏建荣[1] 康熙雄[2] 

机构地区：[1]首都医科大学附属北京友谊医院临床检验中心,北京100050 [2]首都医科大学附属北京天坛医院实验诊断中心,北京100050

出　　处：《首都医科大学学报》2013年第4期535-539,共5页Journal of Capital Medical University

基　　金：国家自然科学基金项目(30972819)~~

摘　　要：目的探讨常见乳酸杆菌与细菌性阴道病(bacterial vaginosis,BV)相关致病菌之间的关系。方法应用16S rRNA PCR检测细菌性阴道病组和非BV组妇女阴道分泌物中3种乳酸菌:卷曲乳酸杆菌、詹氏乳酸杆菌、惰性乳酸杆菌,5种BV相关致病菌:阴道加德纳菌、阴道阿托波菌、普雷沃菌属、纤毛菌属和巨球菌属。结果乳酸杆菌在非BV组的检出率明显高于BV组,BV相关致病菌则相反。詹氏乳酸杆菌与阴道加德纳菌、阴道阿托波菌、纤毛菌属呈负相关;惰性乳酸杆菌与阴道加德纳菌呈负相关;卷曲乳酸杆菌仅与普雷沃菌属呈负相关。3种乳酸杆菌间存在正相关关系。5种BV相关致病菌之间存在正相关关系。乳酸杆菌与BV相关致病菌间存在负相关关系。结论乳酸杆菌之间存在共存关系,BV相关致病菌之间也存在共存关系。Objective To evaluate the association between popular lactobacillus species and bacterial vaginosis （BV） associated bacteria in vaginal flora. Methods Vaginal fluid samples were taken from 170 women, classified as BV （ n = 86 ） or non-BV （ n = 84 ） using the Amsel criteria and Nugent score system. Genomic DNA extracted from these samples was analyzed for bacterial sequences of three Lactobacillus species（L, crispatus, L. jensenii and L. iners ） and five BV associated bacteria （G. vaginalis, A. vaginae, prevotella sp, megasphaera sp and leptotrichia sp） by 16S rRNA PCR. Results The prevalence of Lactobacillus species was significantly higher in the non-BV group than in the BV group, the BV associated bacteria vice versa. Women without L. jensenii were significantly more likely to have G. vaginalis, A. vaginae and leptotrichia sp, without L. iners more likely to have G. vaginalis, without L. crispatus likely to have prevotella sp. Five BV associated bacteria coexisted with each other except that there was no significant association between megasphaera sp and G. vaginalis. Conclusion Not only normal vaginal flora, BV vaginal flora was complicated also because many different BV associated bacteria coexisted . The absence of L. jensenii or L. iners may influence the prevalence of BV associated bacteria.

关 键 词：阴道菌群 细菌性阴道病 乳酸杆菌 分子诊断 

分 类 号：R711.31[医药卫生—妇产科学]