苦马豆素人工抗原免疫小鼠的安全性试验  

Safety Evaluation on Artificial Antigen Swainsonine in Immunized Mice

作　　者：陈根元[1] 王帅[1] 张玲[1] 吴书奇[1] 胡建军[1] 马春晖[1] 

机构地区：[1]新疆生产建设兵团塔里木畜牧科技重点实验室/塔里木大学动物科学学院,新疆阿拉尔843300

出　　处：《湖北农业科学》2013年第14期3370-3372,3377,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31160497);新疆生产建设兵团博士基金专项(2008JC07);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201207)

摘　　要：通过分析苦马豆素(Swainsonine,SW)人工抗原SW-OVA对小鼠机体的影响,进行安全性评价。将36只昆明种小鼠随机分为对照组、试验Ⅰ组、试验Ⅱ组,每组12只。试验组依次进行首免和加强免疫,免疫前采血,分析血清生化指标谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、α-甘露糖苷酶(AMA)、尿素氮(BUN)的变化,血常规指标红细胞数(RBC)、白细胞数(WBC)、淋巴细胞数(LYM)、血小板数(PLT)和血红蛋白含量(HGB)的变化,同时检测免疫组血清中有无SW,并观察小鼠脏器系数及其形态学变化。结果表明,免疫组小鼠与对照组小鼠血清生化指标、血常规指标和脏器系数差异不大,免疫小鼠血清中未检出SW,镜检未见免疫小鼠器官出现中毒现象。合成的人工抗原SW-OVA具有较好的安全性。For evaluating the safety of artificial antigen swainsonine （SW-OVA） in immunized mice, 36 mice were randomly divided into 3 groups; and two test groups were treated by initial immunization and booster immunization. Before each immu- nization, the blood were sampled; and the activity of AST, ALT, AKP, LDH, AMA, BUN in serum as well as the content of RBC, WBC, LYM, PLT, HGB in blood, and swainsonine in serum were analyzed; the pathomorphological changes and coefficient of organ in mice were also observed. The result showed that the differences of serum biochemical indexes blood routine indexes and coefficient of organ between the two immunity groups and control group were not significant （P〉0.05）; and no swainsonine was measured in serum of immunized mice, toxic symptom had not been observed in organ by micro- scopic examination. It was indicated that artificial antigens SW-OVA had good safety to mice.

关 键 词：苦马豆素(Swainsonine SW) 人工抗原 免疫 小鼠 

分 类 号：S852.4[农业科学—基础兽医学]