甘蓝型油菜MYB4基因反义植物表达载体的构建  

Construction of Antisense Plant Expressing Vector of MYB4 Gene of Brassica napus

作　　者：尹锐[1] 柴友荣[2] 

机构地区：[1]吉林农业科技学院生物工程学院,吉林吉林132101 [2]西南大学农学与生物科技学院,重庆400715

出　　处：《湖北农业科学》2013年第14期3428-3430,共3页Hubei Agricultural Sciences

基　　金：国家高技术研究发展计划重大项目(2006AA10A113)

摘　　要：将甘蓝型油菜(Brassica napus L.)MYB4基因家族共保守的467 bp反义片段构建到中间载体pCambia2301G中,替换GUS基因,由CaMV35S启动子驱动,形成了反义植物表达载体,命名为pCambia2301G-MYB4A,并转化到根癌农杆菌(Agrobacterium tumefaciens)LBA4404中形成工程菌株,为进一步研究甘蓝型油菜MYB4基因家族的功能奠定基础。A conserved 467 bp antisense fragment （drived by CaMV35S promoter） of MYB4 genes family of Brassica napus was integrated into intermediate vector pCambia2301G to replace the GUS gene; and the antisense plant expression vector was named as pCambia2301G-MYB4A; and then the recombination vector was introduced into Agrobacterium tumefaciens strain LBA4404 to from an engineering strain. This vector has laid the foundation for further study on the function of MYB4 gene family of Brassica napus.

关 键 词：甘蓝型油菜(Brassica NAPUS L ) 苯丙烷代谢途径 MYB4基因 

分 类 号：S565.4[农业科学—作物学] Q943.2[生物学—植物学]