细菌纤维素高产菌株的筛选和初步鉴定  

Screening and Preliminary Identification of High-Yield Strains of Bacterial Cellulose

作　　者：周胜虎[1] 薛齐佳 刘传凤[1] 黄颖[1] 李丽芬[1] 黎欣[1] 赵静[1] 

机构地区：[1]武汉纺织大学环境工程学院,武汉430073

出　　处：《湖北农业科学》2013年第15期3514-3517,共4页Hubei Agricultural Sciences

基　　金：湖北省教育厅中青年基金项目(20121707)

摘　　要：通过静置富集和分离纯化等步骤从自然腐烂的水果中分离得到6株产细菌纤维素的菌株。从腐烂的芒果中筛选得到1株可产细菌纤维素的混合菌,混合菌产量为湿重617.3 g/L、干重23.9 g/L。经过分离纯化确定该混合菌中只有1株产细菌纤维素菌株M7,在传代培养过程中M7菌株细菌纤维素产量最高且稳定。对M7菌株进行形态特征、生理生化特征和16S rRNA分子序列分析,初步确定M7菌株为葡糖醋杆菌,16S rDNA分子序列已提交至GenBank,序列号为JX303335。6 bacterial cellulose-production strains were isolated from a variety of different types of the natural decay fruits with the step of static enrichment culture, isolation and purification. From mango, one strain of mixed bacteria which can produce the bacterial cellulose was isolated, the yield of mixed bacteria was wet weight 617.3 g/L and dry weight 23.9 g/L, and only M7 strain can produce bacterial cellulose in this mixed bacteria. M7 strain had the highest and stable yield of bacterial cellulose in the course of subcuhuring. M7 strain was initially identified as the glueonacetobacter by analyzing the morphological characteristics, physiological and biochemical characteristics of M7 and determining its 16 S rRNA molecular sequence. The 16 S rRNA molecular sequence was already submitted to the GenBank, and the number of sequence is JX303335.

关 键 词：细菌纤维素 筛选 鉴定 葡糖醋杆菌属 

分 类 号：Q93[生物学—微生物学]