TOLL样受体在疟疾感染小鼠模型中表达量变化规律的研究  

Study on the Change Regularity of Expression Quantity of Toll-Like Receptors in Malaria Infected Mice

作　　者：孙斐[1] 许兵红[1] 杨小林[2] 张佑宏[2] 张海珠[1] 

机构地区：[1]新乡医学院寄生虫学教研室,河南新乡453003 [2]湖北省农业科学院,武汉430064

出　　处：《湖北农业科学》2013年第15期3589-3592,3595,共5页Hubei Agricultural Sciences

摘　　要：为了深入了解疟疾病理过程中TOLL样受体(TLR)和炎症反应的关系,通过Real-time PCR、ELISA等方法,测定了小鼠感染伯氏疟原虫后其TOLL样受体(Toll like receptor,TLR)的mRNA水平和下游炎症因子的血清水平。结果表明,TLR-2、TLR-9、TLR-11的mRNA水平均显著升高,分别为对照的3.0倍、3.5倍和6.0倍。说明宿主通过TLR感知疟原虫的侵入,并启动下游炎症因子的过表达。The relationship between toll-like receptors（TLRs） and inflammation was studied through the detection of the TLR＇s mRNA level and the inflammatory factors level in semen by RT-PCR after the mice was infected with Plasmodium berghei. The results showed that the mRNA level of TLR-2, 9, 11 increased 3.0, 3.5 and 6.0 times of the control group, respec- tively, after 96 hours of infection. It indicated that TLR played an important role in host recognizing of plasmodium invasion and initiated downstream inflammation reactions.

关 键 词：TOLL样受体 REAL-TIME PCR 疟疾 小鼠 

分 类 号：R382.3[医药卫生—医学寄生虫学]