池蝶蚌与三角帆蚌不同组织SOD及EST同工酶的比较研究  

Comparative Studies on SOD and EST Isozymes of Different Tissues between Hyriopsis schlegelii and Hyriopsis cumingii

作　　者：韩庆[1] 鲁密芳[1] 张建平[1] 尹勤勤[1] 杨品红[1] 王文彬[1] 刘良国[1] 

机构地区：[1]湖南文理学院生命科学学院,湖南常德415000

出　　处：《湖北农业科学》2013年第15期3615-3618,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30972260);农业部公益性行业(农业)科研专项(200903028-03);湖南省动物学重点建设学科项目(20117619);湖南省创新团队项目(2008244);湖南省高校动物学重点实验室资助项目(200824632)

摘　　要：采用聚丙烯酰胺凝胶垂直平板电泳法研究池蝶蚌(Hyriopsis schlegelii)和三角帆蚌(Hyriopsiscumingii)5种组织(鳃、外套膜、肠、闭壳肌和斧足)中SOD和EST同工酶的表达情况。结果表明,两种蚌的SOD和EST同工酶均具有明显的组织特异性。SOD、EST酶谱间存在不同程度的种间差异,可作为鉴定池蝶蚌与三角帆蚌的遗传标记。By the vertical polyacryl amide gel electrophoresis （PAGE）, different expressions of SOD and EST isozymes in dif- ferent tissues （gill, mantle,intestinal canal, adductor muscle, and axe foot） of Hyriopsis schlegelii and Hyriopsis cumingii were studied. The results showed that there were significant differences in tissue specificity of the SOD and EST isozymes in both groupers. Some differences in SOD and EST were observed between the two groupers, which can be used as the species ~enetic markers.

关 键 词：池蝶蚌(Hyriopsis schlegelii) 三角帆蚌(Hyriopsis cumingii) 同工酶 电泳 组织特异性 

分 类 号：S917.4[农业科学—水产科学]