甜菜碱醛脱氢酶(BADH)基因转化小麦及其表达  

Transformation of Wheat with a Gene Encoding for the Betaine Aldehyde Dehydrogenase (BADH)

作　　者：郭北海[1] 张艳敏[2] 李洪杰[2] 杜立群[1] 李银心[1] 张劲松[3] 陈受宜[3] 朱至清[1] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]河北省农林科学院粮油作物研究所,石家庄050031 [3]中国科学院遗传研究所,北京100101

出　　处：《Acta Botanica Sinica》2000年第3期279-283,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家"8 63"计划资助项目 !10 1_0 1_0 3_0 1

摘　　要：采用基因枪法将山菠菜甜菜碱醛脱氢酶 (BADH)基因导入小麦 (TriticumaestivumL .)品种 ,并且得以表达。该基因由玉米Ubi1启动子控制。在盐胁迫条件下 ,多数转基因植株叶片的BADH活性比受体亲本提高 1～ 3倍 ,部分植株相对电导率比亲本明显低 ,表明转基因植株的细胞膜在胁迫时有受损较轻倾向。PCR和Southern杂交分析证实外源BADH基因已插入小麦基因组 ,平均转化频率为 4.1%。Betaine aldehyde dehydrogenase ( BADH ) cDNA cloned from Atriplex hortensis L. in the plasmid pABH9 containing maize ubiquitin promoter and bar gene was transferred into wheat (Triticum aestivum L.) by microprojectile bombardment with 4.1% of average frequency of transformation. From 300 young embryo calli bombarded with the plasmid, 24 transgenic plants were obtained showing BADH gene integration by both PCR and Southern blotting analysis. Among the 24 transgenic plants, 13 exhibited higher BADH activity than the control. Some transgenic plants grew normally with healthy roots on the medium containing 0.7% NaCl while the control plants had very poor roots and finally died.

关 键 词：BADH基因 转基因 基因枪 小麦 

分 类 号：Q943.2[生物学—植物学]