蒜瓣鞘表皮组织中肌动蛋白纤丝跨胞分布的共焦荧光显微镜观察  

Confocal Fluorescence Microscopic Observation on the Transcellular Distribution of Actin Filaments in the Epidermis of Garlic Clove Sheath

作　　者：王冬梅[1] 王学臣[2] 张伟成[3] 

机构地区：[1]河北农业大学农学院,保定071001 [2]中国农业大学生物学院,北京100094 [3]中国科学院上海植物生理研究所,上海200032

出　　处：《Acta Botanica Sinica》2000年第3期327-330,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家重点基础研究专项经费资助项目!G19990 1170 0 ;国家自然科学基金!3 973 0 0 5 0

摘　　要：By means of paraformaldehyde fixation, Triton X100 extraction and TRITCphalloidin staining, the presence and distribution patterns of Factin in the outer epidermal cells of the garlic （Allium sativum L.） sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments （AFs） impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of Factin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.By means of paraformaldehyde fixation, Triton X_100 extraction and TRITC_phalloidin staining, the presence and distribution patterns of F_actin in the outer epidermal cells of the garlic (Allium sativum L.) sheath were studied with fluorescence probe technique and confocal laser scanning microscopy. There were a lot of actin filaments (AFs) impenetrate the cell wall, but the AFs with red fluorescence were absent when the cells were treated with cytochalasin D before fixation; the same result was obtained when the cells were treated with unlabeled phalloidin. These results indicate the presence of F_actin in the intercellular channels and that it is related to the plasmodesmata and intercellular trafficking of macromolecules.

关 键 词：胞间连丝 F-肌动蛋白 表皮组织 蒜瓣鞘 显微镜 

分 类 号：Q942[生物学—植物学]