家蚕核型多角体病毒egt基因的结构和功能分析  

The Structural and Functional Analysis of egt Gene of Bombyx mori Nuclear Polyhedrosis Virus

作　　者：季平[1] 何家禄[1] 吕鸿声[1] 吴祥甫[2] 

机构地区：[1]中国农业科学院蚕业研究所农业部家蚕生物技术重点开放实验室,江苏镇江212018 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《病毒学报》2000年第1期54-58,共5页Chinese Journal of Virology

基　　金：国家自然科学基金!( 3 9770 5 75 )

摘　　要：以苜宿银纹夜蛾核型多角体病毒 (AcMNPV)的egt基因作探针 ,从家蚕核型多角体病毒镇江株 (BmNPVZJ- 8)基因组中克隆了egt基因及其旁侧序列 ,作了该片段的酶切图谱 ;测定了BmNPVZJ - 8egt基因序列。与AcMNPV的egt基因相比同源性为 95 % ;基因大小都为 15 18bp。利用egt基因旁侧序列构建了转移载体pUDegt,以绿色荧光蛋白基因 (EGFP)作报告基因取代egt基因 ,构建了由EGFP取代egt基因的重组病毒BmDegt。将含egt基因的BmNPV和不含egt基因的重组病毒BmDegt分别注射感染四龄家蚕幼虫 ,发现BmDegt感染的家蚕个体生长速度缓慢 ,家蚕个体小 。Using egt gene of Autographa californica nuclear polyhedrosis virus(AcMNPV) as probe, egt gene and its flank sequences were cloned from strain BmNPV ZJ 8 genomic DNA. Then their restriction enzyme sites were analyzed and the entire sequencing of BmNPV egt gene was completed. Comparing the sequence of BmNPV egt gene with AcMNPV egt gene, both of their structural gene sequences were 1518bp and their homogeneity was 95%. Using the flank sequences of egt gene, we constructed the transfer vector pUDegt, and EGFP gene was used as report gene to replace egt gene. BmDegt BmNPV with egt gene deleted was constructed. The silkworm larvae infected with BmDegt were compared to those infected with wild BmNPV, they grew up slower , their bodies were shorter and the dying time was moved up.

关 键 词：家蚕核型多角体病毒 EGT 缺失病毒 杀虫效率 

分 类 号：S884.51[农业科学—特种经济动物饲养] Q933[农业科学—畜牧兽医]