中国对虾呼肠孤样病毒感染的电镜观察  

Visualization of Reo-like Virus Infection in Cultivated Penaeus chinensis by Electron Microscopy

作　　者：徐洪涛[1] 屈建国[2] 朴春爱[2] 王文兴[3] 王运涛[1] 相建海[1] 洪涛[2] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国预防医学科学院病毒学研究所,北京100052 [3]国家海洋局第一研究所,山东青岛266003

出　　处：《病毒学报》2000年第1期76-79,共4页Chinese Journal of Virology

基　　金：海洋"8 63" ;中国科学院海洋研究所所长基金;中国科学院EMBL开放课题

摘　　要：In 1996,a serious epizootic called white spot syndrome caused high mortality of cultivated Penaeus chinensis in Qingdao from June to August.Electron microscopy and reinfection test showed that Penaeus chinensis non occluded baculovirus (PcNOBV),the WSBV isolate from P.chinensis in China's Mainland,is the major causative agent of the epizootic.Meanwhile,reovirus like particles were observed in some of the diseased P.chinensis from the following sample tissues:gill,stomach,foregut and ovary.The reovirus like particles could sometimes be found infecting the same shrimp or the same tissue and even the same cell with PcNOBV.The viral particles were spherical with double shelled structure characteristic of reovirus without envelope and located in the cytoplasm of infected tissue cells.The diameter of the particle varies in different tissues ranging from 80 110nm.The size of inner core is 60nm.Based on its morphologic characteristics,the virus is assumed to be a reovirus and named Penaeus chinensis reo like virus(PcREO)temporally.It’s not clear currently what roles does it play in the occurrence of the epizootic.However,the facts of systemic infection of the virus in P.chinensis and dual infection with PcNOBV strongly indicate that PcREO might contribute to the occurrence of the epizootic.Further research is required to determine its characteristics of pathogenicity,biology and molecular biology.In 1996,a serious epizootic called white spot syndrome caused high mortality of cultivated Penaeus chinensis in Qingdao from June to August.Electron microscopy and reinfection test showed that Penaeus chinensis non occluded baculovirus (PcNOBV),the WSBV isolate from P.chinensis in China's Mainland,is the major causative agent of the epizootic.Meanwhile,reovirus like particles were observed in some of the diseased P.chinensis from the following sample tissues:gill,stomach,foregut and ovary.The reovirus like particles could sometimes be found infecting the same shrimp or the same tissue and even the same cell with PcNOBV.The viral particles were spherical with double shelled structure characteristic of reovirus without envelope and located in the cytoplasm of infected tissue cells.The diameter of the particle varies in different tissues ranging from 80 110nm.The size of inner core is 60nm.Based on its morphologic characteristics,the virus is assumed to be a reovirus and named Penaeus chinensis reo like virus(PcREO)temporally.It's not clear currently what roles does it play in the occurrence of the epizootic.However,the facts of systemic infection of the virus in P.chinensis and dual infection with PcNOBV strongly indicate that PcREO might contribute to the occurrence of the epizootic.Further research is required to determine its characteristics of pathogenicity,biology and molecular biology.

关 键 词：中国对虾 呼肠孤病毒 电子显微镜 

分 类 号：S945.463[农业科学—水产养殖]