东北地区大豆根瘤菌遗传多样性与系统发育研究  

Genetic Diversity and Phylogeny of Soybean Rhizobia Isolated from Northeast China

作　　者：王卫卫[1] 关大伟[1] 马鸣超[1] 李力[1] 曹凤明[1] 李俊[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所/农业部农业微生物资源收集与保藏重点实验室,北京100081

出　　处：《大豆科学》2013年第4期433-437,共5页Soybean Science

基　　金：现代农业产业技术体系建设专项资助项目(CARS-04);国家自然科学基金(31200388);中央级公益性科研院所基本科研业务费专项资金(2013-1)

摘　　要：为明确我国东北地区大豆根瘤菌的系统发育地位及主要类群的分布情况,采用BOX-PCR、IGS PCR-RFLP、16SrDNA PCR-RFLP和16S rDNA基因序列分析法对分离自我国东北地区14个地点18个大豆品种的312株大豆根瘤菌及部分参比菌株进行了遗传多样性和系统发育分析。结果表明:供试菌株均为大豆慢生根瘤菌,以Bradyrhizobiumjaponicum为优势种;在IGS PCR-RFLP 72%和16S rDNA PCR-RFLP 76%相似性水平上,供试菌株可分别被分为6和4个群;在BOX-PCR 90%相似性水平上,可将供试菌株分为31个群,表明东北地区慢生大豆根瘤菌具有丰富的遗传多样性。综合考察各种分析结果发现,供试菌株的遗传多样性与其地理来源有一定的相关性,而与大豆品种间相关性不明显。In order to reveal the genetic diversity and phylogeny of soybean rhizobia in the Northeast China, BOX-PCR, 16S rDNA PCR-RFLP, IGS PCR-RFLP and 16S rDNA gene sequencing methods were used to analyze 312 soybean rhizobia isolated from 18 soybean cuhivars in 14 sites in the Northeast China. The results indicated that all the tested rhizobia belonged to Bra- dyrhizobium,of which B. japonicum was the dominant species. Under IGS PCR-RFLP 72% and 16S rDNA PCR-RFLP 76% similarity level, tested rhizobia were divided into 6 and 4 groups, respectively. BOX-PCR fingerprint showed the tested rhizobia were divided into 31 groups under 90% similarity level, which suggested the abundance genetic diversity of Bradyrhizobium in this area. The results suggested that the genetic diversity of tested strains was closely related to the geographical environment, rather than their soybean cuhivar hosts.

关 键 词：大豆根瘤菌 BOX-PCR IGS PCR-RFLP 16S PCR-RFLP 遗传多样性 系统发育 

分 类 号：S565.1[农业科学—作物学] S154.381