潮霉素作为筛选剂对大豆发状根诱导的影响  

Effect of Hygromycin as a Screening Agent on the Induction of Soybean Hairy Roots

作　　者：刘京[1] 刘建巍[1] 韩天富[1] 侯文胜[1] 

机构地区：[1]中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室,北京100081

出　　处：《大豆科学》2013年第4期449-454,共6页Soybean Science

基　　金：转基因生物新品种培育重大专项(2011ZX08010-004)

摘　　要：筛选标记是转基因材料创制中转化细胞存活、生长和发育筛选的基本要素。选用4个不同的大豆基因型,分别以发根农杆菌诱导子叶节产生发状根的数量和根的伸长量为指标,研究了不同质量浓度潮霉素对发状根诱导和根伸长的影响,以期为大豆遗传转化过程中筛选剂的多样性选择及抗潮霉素育种奠定基础。结果表明:潮霉素可以有效抑制非转化细胞的生长,能够用作大豆发状根转化系统中的筛选剂,但不同基因型大豆品种对潮霉素的敏感性存在较大差异。自贡冬豆、吉林小粒1号、吉育47和中黄30在发状根诱导阶段的潮霉素适宜筛选浓度分别为40,16,16,24 mg·L-1;根伸长阶段分别为49,46,33,55 mg·L-1。Selectable marker genes perform a fundamental and essential role in plant transformation by providing the mechanism for the selective survival, growth and development of transformed cells. In the research, four soybean genotypes were selected, and the effects of different concentrations of hygromyein on the hairy root induction and the root elongation were investigated ,with the indicators of number of the hairy roots induced by cotyledons which were infected with the Agrobacterium rhizogenes and the root elongation separately. The purpose was to lay the foundation for the diversity of choices of the selection agent in the soybean genetic transformation process and for the hygromyein resistance breeding. The results showed that, the hygromycin could effectively inhibit the growth of non-transformed cells, successfully act as a successful screening agent in the soybean hairy root transformation system. However, a great difference among different soybean genotypes was exhibited in the sensitivity to hygromycin. The suitable screening concentrations of hygromyein for Zigongdongdou, Jilinxiaoli 1, Jiyu 47 and Zhonghuang 30 at the hairy root induction stage were 40,16,16,24 mg. L^-1 ;and the suitable screening concentrations at the root elongation stage were 49,46,33,55 mg. L ^- 1, respectively.

关 键 词：大豆 基因型 发状根转化 潮霉素 适宜浓度 

分 类 号：S565.1[农业科学—作物学]