冷胁迫对水稻幼苗根系液泡膜质子泵的伤害及钙的调节作用  

Injury of chilling stress to vacuolar proton pumps in roots of rice seedlings and Ca^(2+)regulation

作　　者：赵利辉[1] 刘友良[1] 

机构地区：[1]南京农业大学农学系农业部作物生长调控重点开放实验室,南京210095

出　　处：《南京农业大学学报》2000年第3期5-8,共4页Journal of Nanjing Agricultural University

基　　金：江苏省联建重点学科专项科研基金(9701)资助

摘　　要：选用耐冷性不同的两个水稻品种 ,研究了在低温下幼苗根系活力、液泡膜质子泵活性的变化及外源钙的调节作用。结果表明 ,8℃低温处理的水稻幼苗根系活力降低 ,随着胁迫时间的延长 ,伤害进一步发展 ,抗冷性弱的汕优 6 3比抗冷性强的武育粳 3号伤害严重。 8℃处理 7d内 ,两品种根系V ATPase (液泡膜H+ ATP酶 )和汕优 6 3V PPase (液泡膜H+ 焦磷酸酶 )活性持续下降 ,而武育粳 3号V PPase在前 3d内活性上升 ,5d后才呈下降态势。根系在低温下维持较高的V PPase活性 ,可能是水稻耐冷的原因之一。 8℃低温处理外加 2 0～ 40mmol/LCa (NO3) 2Response of vacuolar proton pumps in the roots of rice seedlings to chilling stress and Ca 2+ regulation were investigated.The results showed that the root activity and V?ATPase activity in both Shanyou 63(chilling?sensitive)and Wuyujing 3(chilling?insensitive)roots decreased with time of chilling(8 ℃)treatment.The V?PPase specific activity in Wuyujing 3 for the first 3 days of chilling increased,and began to decrease after 5 days of chilling.The results indicated that the relative high V?PPase activity in the roots at low temperature may be one of factors in rice chilling?tolerance.The root activity in rice seedlings at 8 ℃were improved by exogenous 20?40 mmol/L Ca 2+

关 键 词：水稻 冷害 液泡膜 H^(+)-ATP酶 H^(+)-焦磷酸酶 Ca^(2+) 

分 类 号：S511.01[农业科学—作物学] S435.111.3