豫医卷毛大鼠表皮生长因子基因的比较生物学分析  

Comparative biology analysis of EGF gene in Yuyi curly hair rats

作　　者：史崇敏[1] 张明昊[2] 朱奎成[1] 杜春燕[1] 王君敏[1] 胡涛[1] 薛敬礼[1] 章金涛[1] 

机构地区：[1]郑州大学实验动物中心,郑州450052 [2]河南中医学院医学机能实验室,郑州450046

出　　处：《郑州大学学报（医学版）》2013年第4期447-450,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目31071923

摘　　要：目的:对豫医卷毛大鼠的表皮生长因子(EGF)基因进行比较生物学分析。方法:采用PCR方法对豫医卷毛大鼠EGF基因cDNA序列进行克隆测序,并与BN大鼠、家鼠、原鸡、猕猴和人EGF基因的核苷酸序列及其编码蛋白的氨基酸序列进行同源性比较和系统遗传学分析。结果:获得了豫医卷毛大鼠EGF基因全长3522bp的cDNA序列(GenBank登录号:JX437943)。豫医卷毛大鼠EGF基因编码区的核苷酸序列与BN大鼠、家鼠、原鸡、猕猴和人的同源性分别为97.8%、83.4%、59.7%、76.5%、73.5%,其编码蛋白的氨基酸序列同源性分别为99.3%、79.9%、52.9%、69.6%、69.4%。结论:EGF基因在进化过程中具有一定的保守性,但不同物种之间也具有特异性。Aim： To study the difference of epidermal growth factor（ EGF） gene and its coding protein between Yuyi curly hair rats and others species.Methods： PCR technique was developed to clone the sequence of cDNA of EGF gene from Yuyi curly hair rats,and biologic characters of nucleotide and amino acid sequence encoding EGF gene in different species （ Norway rats,house mice,chicken,rhesus monkey,and human） were analyzed.Results： A full-length cDNA of Yuyi curly hair rats was 3 522 bp （ Accession Number： JX437943） .The EGF gene nucleotide homology of Yuyi curly hair rats to Norway rats,house mice,chicken,rhesus monkey,and human was 97.8% ,83.4% ,59.7% ,76.5% ,and 73.5% ,and that of amino acid sequences was 99.3% ,79.9% ,52.9% ,69.6% ,and 69.4% ,respectively.Conclusion： A certain degree of conservation of EGF gene exists among different mammalian taxons,but there were some specificity among different mammalian taxons.

关 键 词：卷毛大鼠 表皮生长因子 同源性比较 

分 类 号：Q953[生物学—动物学]