大鼠丘脑中央下核GABA纤维终末与腹外侧眶皮质投射神经元的突触联系  

Synaptic connections between GABA terminals and neurons projecting to ventrolateral orbital cortex in rat thalamic nucleus submedius

作　　者：罗阿丽 王唯析[2] 强辉[3] 靳辉[2] 陈国敏[2] 

机构地区：[1]西安市结核病胸部肿瘤医院病理科,西安710061 [2]西安交通大学医学院人体解剖与组织胚胎学系,西安710061 [3]陕西省人民医院骨科,西安710068

出　　处：《郑州大学学报（医学版）》2013年第4期466-469,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：陕西省自然科学基金资助项目2006C205

摘　　要：目的:观察大鼠丘脑中央下核(Sm)GABA纤维终末与腹外侧眶皮质(VLO)投射神经元的突触联系。方法:18只大鼠麻醉后固定于脑立体定位仪上,于VLO微量注射辣根过氧化物酶(HRP),48h后,应用包埋前HRP组化和GABA免疫组化双重标记电镜技术观察大鼠SmGABA纤维终末与VLO投射神经元的突触联系。结果:光镜下Sm内有大量GABA纤维终末及少数阳性神经元,电镜下可见GABA纤维终末与HRP标记的阳性神经元胞体及树突间构建了对称性和(或)非对称性突触。结论:形态学上证实了Sm内GABA纤维终末与VLO投射神经元胞体及树突发生突触联系。Aim： To study the synaptic connections between GABA ergic terminals and the neurons which project to the ventrolateral orbital cortex（VLO） in the rat thalamic nucleus submedius（Sm） . Methods： Eighteen rats were fixed in the stereotaxic apparatus after anesthesia. The Horse radish peroxidase-retrograde tracing method was used in VLO. After 48 h,the double-labeling immunohistochemical electron microscopic technique（pre-embedding histochemical detection of HRP combined with pre-embedding GABA-immunohistochemical technique） was used to observe the synaptic connections between GABA ergic terminals and the neurons which project to the VLO in Sm. Results： Under optical microscope,in Sm,there were large number of GABA positive terminals and few positive neurons. Under electron microscope,the symmetrical and（or） asymmetrical synaptic connections between GABA positive terminals and HRP-retrograde tracing labeled cell bodies and dendrites were observed. Conclusion： GABA positive terminals make synaptic connections with the projection neuron bodies and dendrites from Sm to VLO.

关 键 词：丘脑中央下核 突触 GABA 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]