利用聚己内酯/羟基磷灰石复合支架修复犬胸骨缺损  

Individualized Healing in a Canine Partial Sternal Defect Model by a Polycaprolactone/Hydroxyapatite Scaffold

作　　者：禤艺文[1] 刘腾飞[1] 李晓亮[1] 肖燎原[2] 徐志飞[1] 

机构地区：[1]第二军医大学附属长征医院胸心外科,上海200003 [2]第二军医大学附属长征医院肾内科,上海200003

出　　处：《现代生物医学进展》2013年第22期4214-4218,4225,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金-青年基金项目(31100691);上海市科委重点基金项目(10411955800)

摘　　要：目的:探讨利用生物可降解支架修复动物胸骨缺损,为临床手术治疗提供新的可行性方法。方法:对于12只比格犬进行手术切除部分胸骨,并利用聚己内酯/羟基磷灰石(PCL/HA)复合支架,并制备出与临床相似的胸骨缺损模型。实验动物分成2组,分别是:空白对照组和PCL/HA支架组。分别于术后第4、12周进行胸部CT扫描,并对胸廓进行三维重建,观察胸骨缺损部位的修复情况,并在第12周取胸骨缺损部位组织进行硬组织切片,苦味酸-品红染色,观察缺损部位的骨组织修复情况,并利用软件进行骨组织比率分析,评估修复情况。结果:通过检查发现空白对照组的胸骨缺损部位未见明显骨连接,胸廓的骨性结构有明显畸形,PCL/HA支架组能很好地维持胸廓的完整性,组织学检查发现PCL/HA支架组的缺损部位有明显新生骨形成,通过软件分析可发现支架组的骨组织比率较空白组的高(P<0.05)。结论:这些结果表明采用PCL/HA复合材料支架能很好地修复胸骨缺损。Objective： To investigate the individualized reconstruction of partial sternal defects with new biodegradable material in a large animal model.Methods： The composite polycaprolactone/ hydroxyapatite（PCL/HA） tissue scaffolds with specific grooves manufactured by the technique of fused deposition modeling（FDM） incorporate patient-specific information was used to repair the sternal defect.The defects were surgically created in a second or third sternocostal joint（including partial sternum） of eighteen Beagle dogs.The animals were divided into two groups（n=6）： Blank group and PCL/HA group.Radiographic examination,histological and histomorphometric analyses were performed to evaluate the result.Results： In the blank group,there was no bone union in the defect and junctions,and the defect site couldn＇t maintain the original cosmetic integrity and it was easily distorted.In the PCL/HA group,the scaffolds retained their shape without significant degradation at 12 weeks.There was new bone formation and bone-union in the PCL/HA group by radiographic and histological examination.And PCL/HA would be more mineralized tissue area at implant sites（P0.05）.Conclusion： These results reveal that manufacture the PCL/HA scaffolds with specific grooves by using the technique of FDM could repair the sternal defect satisfactorily.

关 键 词：聚己内酯 羟基磷灰石 胸骨重建 组织工程 

分 类 号：Q813[生物学—生物工程]