转基因MSC对放射损伤胸腺组织修复的研究  

Study on the Tissue Repair of Mice's Thymus Injured by Radiation with Transgenosis MSC

作　　者：宁昌[1] 左洪莉[2] 胡锴勋[2] 余长林[2] 刘广贤[2] 

机构地区：[1]解放军第161医院肿瘤科,湖北武汉432100 [2]军事医学科学院附属307医院血液科,北京100071

出　　处：《现代生物医学进展》2013年第22期4233-4236,4321,共5页Progress in Modern Biomedicine

摘　　要：目的:探讨转基因MSC能否修复放射损伤的胸腺。方法:雌性BALB/C小鼠120只,随机分为4组:对照组30只,予以假照射;照射组30只,60Coγ射线胸腺单次照射,剂量为9Gy;照射+MSC组30只,9Gy照射后2小时输注转基因MSC;对照+MSC组30只,假照射后2小时输注转基因MSC。照射后1、7、14、21、28天检测各组小鼠血像、胸腺指数及胸腺病理。结果:照射+MSC组血像和胸腺指数在各个时间点均优于照射组,胸腺病理显示组织结构恢复明显加快。结论:转基因MSC能归巢至放射损伤胸腺并能促进胸腺的修复,为干细胞的临床应用提供了实验依据。Objective： To investigate whether gene modified MSC can repair thymus injured by radiation.Methods： Female BALB/C mice were randomly classified into four groups,irradiation group were irradiated with doses of60Co γ-rays 9 Gy,control group received sham-irradiation,irradiation＋MSC group were irradiated with doses of 9 Gy and injected MSC 2 h later,contorl＋MSC group received sham-irradiation and injected MSC 2 h later.The thymus index,thymus pathology and peripheral blood of each group was detected on the 1st,7th,14th,21st,28thday after irradiation.Results： Compared with the irradiation group,the thymus index,thymus pathology and peripheral blood of the irradiation＋MSC group was more superiority and the differences was significantly.Conclusion： Gene modified MSC could recruite to the injured thymus and participated in the repairation of the thymus.

关 键 词：转基因 间充质干细胞 胸腺 放射性损伤 组织修复 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] Q78[医药卫生—基础医学]