不同剂量X射线对大鼠精子CRISP2 mRNA表达水平的影响  

Effects of X-ray Irradiation on the Expression Level of CRISP2 mRNA in Spermatogenesis of Male SD Rats

作　　者：李仲辉[1,2] 许羽龙[2] 左超[2] 邹练[1,2] 江其生 李峰生 王思念 李晓 

机构地区：[1]山西医科大学研究生学院,山西太原030001 [2]解放军第二炮兵总医院泌尿外科,北京100088 [3]解放军第二炮兵总医院中心实验室,北京100088

出　　处：《现代生物医学进展》2013年第23期4424-4428,共5页Progress in Modern Biomedicine

基　　金：全军医药卫生科研基金项目(06G016)

摘　　要：目的:观察不同剂量X射线对大鼠精子CRISP 2 mRNA表达水平的影响,探讨其在电离辐射所致大鼠精子功能改变中的作用。方法:用吸收剂量为1、2、4、和6 Gy的X射线分别照射活体SD大鼠的外生殖系统1、4、8、12、24h后,用PCR技术检测精子CRISP2基因mRNA表达水平;用光学显微镜观察精子活力。以未照射组为对照。结果:4、6 GyX射线照射不同时间(1、4、8、12、24h时)后大鼠精子的CRISP 2 mRNA相对表达量均较对照组显著下降(P<0.05),其中6 Gy照射24小时后相对表达量最低(P<0.01),而4Gy照射组与6Gy照射组相比较差异无统计学意义(P>0.05);2 Gy X射线照射8h后CRISP2 mRNA相对表达量下降有统计学意义(P<0.05);2 GyX射线照射1、4h后及1GyX射线照射不同时间(1、4、8、12、24h)后大鼠精子的CRISP 2 mRNA相对表达量较对照组下降,但差异无统计学意义(P>0.05)。1、2GyX射线照射不同时间(1、4、8、12、24小时)及4GyX射线照射(1、4、8h)后,精子活力与正常对照组相比无明显改变(P>0.05);4GyX射线照射12、24h后大鼠精子活力显著低于正常对照;6GyX射线照射不同时间(1、4、8、12、24h)后,精子活力明显低于对照组(P<0.05)。结论:不同剂量X射线照射不同时间可导致SD大鼠精子活力下降,这可能与其下调CRISP2基因的mRNA表达水平有关。Objective： To observe the impact of expression levels of sperm CRISP2 mRNA with different dose and time-effects of x-ray irradiation on the rats. To explore the changes of sperm function of rats in ionizing radiation. Methods： The external reproductive system of SD rats were exposed to x-rays with the dose of 1,2,4 and 6 Gy respectively. The untreated rats were defined as the control group. The relative levels of CRISP2 mRNA expression by PCR and the motility （a ＋ b %） by optical microscope observation at 1,4,8,12 and 24 h after irradiation in sperm. Results： The levels of rats＇ sperm CRISP2 mRNA exposed by 4 or 6 Gy x-rays group was significantly lower than that of the control group （P〈0.01）; When accepted 2 Gy by irradiation, the levels of rats＇ CRISP2 mRNA have reduced to the different degree, but significant changes occured in exposure after 8 h （P〈0.05）; There was no change of CRISP2 mRNA with lower dose （1Gy）（P〉0.05）. There was no significantly decrease in 1,2 Gy and 4 Gy （P1,4,8）exposure sperm motility group （P〉0.05）; 4 Gy（12,24h）6 Gy exposure group sperm motility is obviously lower than that of the control group （P〈0.05）. Conclusion： Under certain condi- tions, dif- ferent dose X-ray irradiation at differ- ent times can lead to SD rats decreased sperm motility, which may be associated with down CRISP2 gene mRNA expression levels.

关 键 词：X射线 精子 CRISP 2 MRNA 

分 类 号：Q95-3[生物学—动物学] Q691