巢式PCR法在早期梅毒诊断中的应用评价  

Evaluation of nPCR for Detection of Treponema pallidum in Primary Syphilis

作　　者：雷震山 杜剑强 吴少琴 张勤国 尹云华 

机构地区：[1]深圳市保健办,广东深圳518020

出　　处：《现代生物医学进展》2013年第23期4529-4531,4590,共4页Progress in Modern Biomedicine

摘　　要：目的:评价巢式PCR(nPCR)法在早期梅毒诊断中的临床应用价值,以提高早期梅毒诊断的灵敏度和特异性。方法:选择2010年10月至2011年11月来我院就诊,经临床综合分析为一期梅毒的患者195例和同期就诊的120例非梅毒患者为研究对象,采用nPCR法对棉拭子标本和血液标本中梅毒螺旋体特异性基因tpp47进行扩增检测,所有标本同时做暗视野镜检和Tp-ELISA血清学检测。结果:nPCR法共检测出阳性标本176例,其灵敏度和特异性分别为90.3%和100%,明显高于暗视野镜检和Tp-ELISA法,差异有统计学意义。结论:nPCR法在早期梅毒诊断中具有较高灵敏度和特异性,可以作为暗视野镜检和血清学检测的补充试验。Objective： To evaluate the clinical value of nPCR in detection of primary syphilis and improve the sensitivity and specificity in diagnosis of primary syphilis. Methods： 195 cases of primary syphilis hospitalized from October 2010 to November 2011 and 120 cases of non syphilis at the same term were selected, tpp47 gene of Treponema pallidum from swabs and blood specimens were detected by nPCR assay. Results： 176 of 195 patients had positive nPCR results, with a sensitivity of 90.3% and a specificity of 100%, which were significantly higher than those detected by dark-field microscopy or Tp-ELISA. Conclusion： The nPCR method had higher sensitivity and specificity in the diagnosis of primary syphilis., which was a useful addition to serology and dark-field microscopy for the diagnosis of primary syphilis.

关 键 词：梅毒螺旋体 巢式PCR 早期梅毒 tpp47 

分 类 号：R759.11[医药卫生—皮肤病学与性病学]