卡介苗稳定L型对动物致病性的研究  

Study on pathogenicity of stable L-form derived from BCG in Animals

作　　者：李丽[1] 丁元廷[1] 

机构地区：[1]贵阳中医学院第一附属医院检验科,贵州贵阳550001

出　　处：《贵州医药》2013年第1期12-15,共4页Guizhou Medical Journal

摘　　要：目的探讨卡介苗稳定L型菌对动物的致病作用。方法将卡介苗稳定L型纯培养物注射至PPD试验阴性豚鼠的腹部皮下,观察动物的发病情况、PPD反应、组织病理学改变以及分离培养和鉴定卡介苗及其L型。结果2×108个/mL剂量的卡介苗稳定L型感染动物21d后可见局部皮肤轻度红肿及腹股沟淋巴结肿大,第4周各感染动物的PPD试验仍为阴性。组织病理学检查可见非特异性增生性炎症反应,未见结核结节和干酪样坏死。于感染31d后组织切片抗酸染色可发现抗酸阳性的圆球体,阳性率为20%。分离培养未检出卡介苗及其L型。结论卡介苗稳定L型可在动物局部组织中存活并且具有引起局部组织非特异性炎性病理损害的作用,但不能引起动物形成PPD阳性反应。Objective To probe the pathogenicity of stable L-form derived from Bacillus CalmetteGuerin （ BCG ） to animals. Methods The guinea-pigs were infected with the pure cultures of the stable L-form derived from BCG and then skin reaction, swell of inguinal lymphaden, tuberculin reaction and tissue pathological change of the animals were observed. The bacterial forms and the L-forms of BCG were isolated respectively from the tissue samples of the animals. Results The redness and swelling of skin, and the swollen inguinal lymphaden were observed in the guinea-pigs infected with the stable L- form cells of the number of 2× 108 /ml until 21th day of infection, but the tuberculin purified protein derivative （PPD） reactions were always negative after 4 weeks of infection. The nonspeeifie proliferative inflammatory reaction would be found but the tuberculosis tubercle and caseation necrosis could not be found by histopathological examination. By acid fast staining, the positive reaction of the L- spheroplasts could be found （positive rate of 20%） in pathogenic tissue samples until 31th day of in- fection. The BCG and their L-forms could not be found by isolation. Conclusion The stable L-form de- rived from BCG could survive and still had pathogenicity in local tissue of guinea-pig, where nonspecific inflammation pathological lesion could be found, but its virulence is weaker than that of the parent bacteria, and the PPD reactions were negative.

关 键 词：卡介苗 细菌L型 动物 

分 类 号：R378.91[医药卫生—病原生物学]