用生物信息学方法预测2-型猪链球菌保护性抗原IBP的B细胞表位  

Prediction of B cell epitope for protective antigen IBP against Streptococcus suis serotype 2

作　　者：姜楠[1] 黄悦[1] 李红[1] 胡蓉[1] 苏敏[1] 

机构地区：[1]贵阳医学院组胚教研室,贵州贵阳550004

出　　处：《贵州医药》2013年第1期23-25,共3页Guizhou Medical Journal

摘　　要：目的用多参数预测SS2保护性抗原IBP的B细胞表位。方法以2-型猪链球菌(SS2)保护性抗原IBP推定的氨基酸序列为基础,采用Kyte-Doolittle法分析蛋白质的亲水性,Emini法预测蛋白质的表面可能性,Jameson-Wolf法预测蛋白质的抗原指数;辅以Garnier-Robson法、Chou-Fasman法和Karplus-Schulz法对IBP蛋白二级结构中柔性区域的分析,预测IBP的B细胞表位。结果结果表明,推测IBP的B细胞表位位于IBP蛋白N-端第14～18、95～108、133～137、172～179、191～198、205～208区域。结论通过预测SS2保护性抗原IBP的B细胞表位,可望为实验研究SS2的多表位疫苗奠定基础。Objective To study the prediction of the B cell epitopes for protective antigen IBP against S. suis 2 based on multiple parameters. Methods he secondary structure flexible regions of protective antigen IBP against Streptococcus suis serotype 2 （S. suis 2） was analyzed by the Gamier-Robson＇s method, the Chou-Fasman＇s method and the Karplus-Schulz：s method based on its putative amino sequence. And hydrophilicity plot, surface probability plot and antigenicity index were obtained by the methods of Kyte-Doolittle, Emini and Jameson-Wolf, respectively. Results Combined with these results, B cell epitopes for IBPmwere predicted at the N-terminal No. 14-18, 95-108, 133-137, 172 -179, 191-198, 205-208. Conclusion Taken together, prediction of the B cell epitopes for protective antigen IBP against S. suis 2 based on multiple parameters would lay foundation on experimental re- search of multi-epitope vaccine against S. suis 2.

关 键 词：2-型猪链球菌 保护性抗原 B细胞表位 

分 类 号：S852.61[农业科学—基础兽医学] R446.62[农业科学—兽医学]