贵阳地区汉族人群人类血小板抗原1~5,15基因多态性与2型糖尿病合并周围神经病变的相关性研究  

Study on the association between human platelet antigen-1~5,15polymorp-hism and type 2 diabetes with peripheral neuropathy in Guiyang city

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作  者:张亚华[1] 徐时方[1] 洪欢山[1] 郑婧[1] 徐淑静[1] 尹红[1] 范良敏[1] 

机构地区:[1]贵阳医学院附属医院急诊科,贵州贵阳550004

出  处:《贵州医药》2013年第6期487-491,共5页Guizhou Medical Journal

基  金:贵阳市科技局基金资助项目[编号(2012)24]

摘  要:目的探讨人类血小板抗原1~5,15(HPA-1~5,15)基因多态性与贵阳市汉族2型糖尿病合并周围神经病变的发生是否具有相关性。方法 2型糖尿病合并周围神经病变患者75例(DPN组),不合并周围神经病变患者24例(非DPN组),健康体检者100例(对照组)。采用酚/氯仿方法提取DNA,用多聚酶链反应-序列特异性引物方法进行基因组分型。结果 DPN组、非DPN组的HPA-1aa、2aa、3aa型基因频率均明显高于对照组,而DPN组与非DPN组之间差异无显著性;DPN组、非DPN组的HPA-1a、2a、3a等位基因频率明显高于对照组,而DPN组与非DPN组之间差异无显著性。HPA-4、5、15的基因型、等位基因频率在对照组、DPN组与非DPN组之间比较差异均无显著性。结论 HPA-1~5,15基因多态性与贵阳市汉族人群2型糖尿病合并周围神经病变的发生可能无关。Objective To study the distribution of human platelet antigen (HPA, HPA-1-5, 15) gene polymorphisms in Han people from Guiyang City, and observe whether the genetic risk factors occur in type 2 diabetes patients with peripheral neuropathy. Methods Seventy-five cases of type2 dia- betes mellitus patients with peripheral neuropathy(DPN group), 24 cases of type2 diabetes mellitus patients without peripheral neuropathy(non-DPN group)and 100 healthy subjects(control group)were enrolled in this study. DNA was extracted via phenol/chloroform method and the genotypes were de- termined by polymerase chain reaction-sequence specific primer (PCR-SSP). Results HPA-laa, 2aa, 3aa gene frequencies of DPN group and non- DPN group were significantly higher than that of the con- trol group, and there were no significant differences between the DPN group and the non-DPN group. The HPA-1a,2a, 3a allele frequencies of DPN group and non- DPN group were significantly higher than that of the control group, while there were no significant differences between the DPN group and the non-DPN group. The genotype and gene frequencies of HPA-4, 5, 15 between any two of the three groups have no significant differences. Conclusion The HPA -1-5, 15gene polymorphisms may he not correlated with type2 diabetes mellitus patients with peripheral neuropathy in Han population of Guiyang City.

关 键 词:人类血小板抗原 基因多态性 2型糖尿病 周围神经病变 

分 类 号:R34[医药卫生—基础医学] R587.1

 

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