一株腐殖质还原功能菌的分离筛选与鉴定  

Isolation and Identification of a Humic Substances Bacterial Strain

作　　者：陈楠[1,2,3] 邓晓[1,3] 邹雨坤[1,3] 武春媛[1,3] 李勤奋[1,3] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]海南大学环境与植物保护学院,海南海口570228 [3]农业部儋州农业环境科学观测实验站,海南儋州571737

出　　处：《华北农学报》2013年第4期179-183,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金资助项目(41101477);国家科技支撑计划子课题项目(2012BAC18B04-5);海南省重点科技项目(ZDXM20100021)

摘　　要：腐殖质呼吸是一种新型微生物能量代谢方式,菌种资源研究成为腐殖质呼吸的研究重点。利用厌氧富集分离方法,从木薯堆肥土壤中获得1株具有腐殖质还原特性的兼性厌氧菌株,编号为HN02。HN02为革兰氏阳性菌,细胞为短杆状,菌落呈黄色,表面光滑,边缘整齐;最适生长盐度为1%,最适生长温度为30℃,最适生长pH值7.5～8.5;可利用麦芽糖作为碳源,明胶液化和硝酸盐还原为阴性;16S rDNA基因序列分析结果表明:菌株HN02与天花板节杆菌亲缘关系最近,同源性达99.4%;HN02能在以葡萄糖作为电子供体,以AQDS为唯一电子受体的厌氧环境下将AQDS还原为AH2QDS,还原率达40%。菌株HN02是1株具有腐殖质还原特性的天花板节杆菌,首次报道该菌属菌株具有腐殖质还原特性,为完善腐殖质还原特性的微生物系统提供理论基础。The humus respiration is a new way of microbial energy metabolism.And the research about strains resources becomes the focus point.A facultative anaerobic humus-reducing bacterium,designated HN02,was isolated from cassava dregs composting.Strain HN02 is a gram-positive,short rod-shaped,gelatin hydrolysis and nitrate reduction-negative bacterium.Optimal growth salt was 1%（NaCl）;optimal growth temperature was 30 ℃ and optimal pH was at 7.5-8.5.Analysis of 16S rDNA of HN02 indicated that the closest phylogenetic relative among the valid species was Arthrobacter tecti,with 99.4% 16S rDNA similarity.Strain HN02 can utilize AQDS via anaerobic enrichment procedure with glucose as the electron donor and AQDS as the sole terminal electron acceptor.The reduction ratio of AQDS was 40%.Thus,the humus-reducing of Arthrobacter tecti HN02 is here reported for the first time and it can provides the theoretical basis for microbial systems with characteristics of humus reduction.

关 键 词：腐殖质 还原菌 节杆菌属 

分 类 号：S432.4[农业科学—植物病理学]