分子标记辅助选择定向改良武运粳7号的条纹叶枯病抗性  

Improving Resistance of Japonica Variety Wuyunjing 7 to Rice Stripe Virus Disease by Molecular Marker-assisted Selection

作　　者：姚姝[1] 陈涛[1] 骆名瑞[1] 张亚东[1] 朱镇[1] 赵庆勇[1] 周丽慧[1] 王才林[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《华北农学报》2013年第4期195-203,共9页Acta Agriculturae Boreali-Sinica

基　　金：转基因生物新品种培育重点项目(2009ZX08001-019B);江苏省农业科技自主创新基金项目(CX(11)1022);江苏省科技支撑计划项目(BE2012303)

摘　　要：利用分子标记辅助选择技术改良武运粳7号的条纹叶枯病抗性,培育抗条纹叶枯病的粳稻新种质。以日本优质粳稻品种关东194为条纹叶枯病抗性基因Stv-bi的供体,采用定向回交育种策略,改良高产、感病品种武运粳7号的条纹叶枯病抗性。在连续回交和自交过程中,利用与Stv-bi紧密连锁的SCAR标记进行跟踪检测,并结合条纹叶枯病田间抗性鉴定以及农艺性状的系统选择,将抗性基因Stv-bi转移到武运粳7号中。采用分子标记辅助选择,从BC3F3世代中筛选出14个具有Stv-bi纯合基因型的改良株系。其综合性状与武运粳7号十分相近,有的性状甚至优于武运粳7号。利用定向回交和分子标记辅助选择相结合的技术体系,可显著提高抗性改良的预见性和准确性,缩短育种年限、加快育种进程。新育成的武运粳7号抗性改良系,为条纹叶枯病抗性育种提供了重要的遗传资源。To develop new germplasms resistant to rice stripe disease（RSV）,the molecular marker-assisted selection（MAS）strategy was applied to improve the resistance of Wuyunjing 7.As the donor parent of resistance gene Stv-bi,the japonica rice Kantou 194 with high quality was used to improve the RSV resistance of Wuyunjing 7 with high yield but susceptible to RSV by the breeding strategy of directional backcross.In continuous backcross and selfcross generations,the SCAR（Sequence characterized amplified region）marker tightly linked with Stv-bi was used for MAS,by combining with the identifications of field resistance to RSV and agronomic traits analysis.And finally,resistance gene was successfully transferred into Wuyunjing 7.14 improved rice lines carrying the disease-resistance gene were obtained from BC3F3 generation by molecular marker selection.These lines were the same as and even better than donor parent in comprehensive characters,which retained the characteristics of high yield and enhanced obviously the resistance to rice stripe virus.Utilization of technology systems intergrating directional backcross and MAS,the predictability,accuracy and efficiency of breeding for disease resistance could be improved prominently.Meanwhile,these RSV-resistant rice lines,newly developed could provided important genetic resources for the future resistance breeding.

关 键 词：水稻 条纹叶枯病 Stv-bi基因 回交育种 分子标记辅助选择 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]