剑麻内生细菌JM-3对苹果腐烂病抑制作用的研究  

Inhibiting Effects of Endophytic Bacterial Strain JM-3 from Agavesisalana Perrineagainst Valsa ceratosperma

作　　者：张淑颖[1,2] 曲田丽[3] 孙阳[4] 董向丽[2] 王彩霞[2] 李保华[2] 肖春[1] 

机构地区：[1]云南农业大学植保学院,云南昆明650201 [2]青岛农业大学农学与植保学院,山东青岛266109 [3]青岛农业大学化学与药学院,山东青岛266109 [4]青岛农业大学生科院,山东青岛266109

出　　处：《华北农学报》2013年第4期208-213,共6页Acta Agriculturae Boreali-Sinica

基　　金：现代农业产业技术体系项目(CARS-28);山东省科技攻关计划项目(2010GNC10918);国家自然科学基金项目(31000891;31272001);山东省"泰山学者"建设工程项目

摘　　要：从剑麻叶片组织内分离获得6株内生细菌,其中JM-3菌株对离体苹果枝条上苹果腐烂病菌的扩展致病有较好的抑制效果。JM-3菌株在培养过程中能产生抑菌物质,其无菌发酵滤液能抑制腐烂病菌菌丝的生长和分生孢子的萌发,导致菌丝和芽管膨大畸形,最终导致菌丝破裂、原生质外流。JM-3菌株对多种果树和农作物病原真菌菌丝的生长都有明显的抑制作用。经16S rDNA序列分析和RiboPrinter分析,鉴定菌株JM-3为枯草芽孢杆菌。Six isolates of endophytic bacterial strains were isolated from healthy leaves of Agavesisalana Perrine,in which the strain JM-3 inhibited canker lesion expansion of Valsa ceratosperma on excised twigs significantly.The cell-free fermentation liquid of JM-3 inhibited mycelium growth and conidial germination of Valsa ceratosperma.The isolates produced anti-fungi substances,which caused malformation of mycelia and germtubes.The malformed mycelium burst and protoplasm flow-out finally.JM-3 also showed clear inhibiting effect against mycelial growth of pathogen from several fruit trees and crop.JM-3 was identified as Bacillus subtilis based on 16S rDNA sequencing and RiboPrinter results.

关 键 词：内生细菌 枯草芽孢杆菌 苹果腐烂病 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]