家兔ATG16L1基因多态性与非特异性消化道紊乱易感性的研究  

The Association between the Polymorphism of ATG16L1 and Non-specific Digestive Disorder in Rabbit

作　　者：肖正龙[1] 张龚炜[1] 杨宇[1] 王杰[1] 赖松家[1] 

机构地区：[1]四川农业大学动物遗传育种研究所,四川成都611130

出　　处：《华北农学报》2013年第4期234-238,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家现代农业技术体系项目(CARS-44-A-2);四川省"十二五"科技支撑项目(2011NZ0099-4)

摘　　要：为了研究家兔ATG16L1基因对非特异性消化道紊乱的易感性,试验采用PCR-HRM技术,首次对家兔ATG16L1基因进行单核苷酸多态性(SNP)检测,同时结合低纤维群体结肠ATG16L1基因mRNA表达量进行关联分析。结果表明:ATG16L1基因存在1个SNP位点(c.1482 G>A)与NSDD的易感性相关联;c.1482 G>A位点A等位基因能增加NSDD的易感性(OR=1.43,95%置信区间:1.08～1.91,P<0.05);在隐性遗传模型中,AA基因型能增加NSDD的易感性(OR=1.69,95%置信区间:1.05～2.78,P<0.05);ATG16L1基因在结肠中的mRNA表达量随着炎症的加剧显著升高(P<0.05);AA基因型在整个低纤维诱导NSDD组中的表达水平最低(P<0.05)。这些结果均表明,ATG16L1基因与家兔NSDD的遗传易感性相关。To study the susceptibility of ATG16L1 gene to non-specific digestive disorder（NSDD） in rabbit,single nucleotide polymorphism（SNP） of rabbit ATG16L1 gene was firstly detected by PCR-HRM and the association analysis of ATG16L1 gene mRNA expression in colon of fibre-deficient diet group was conducted.The results found that there was a SNP locus（c.1482 G〉A） in ATG16L1 gene and the SNP locus was associated with the susceptibility to NSDD;the allele A of c.1482 G〉A could increase the susceptibility（OR = 1.43,95% confidence interval：1.08-1.91,P〈0.05）;in recessive genetic model,the genotype AA could increase the susceptibility（OR = 1.69,95% confidence interval：1.05-2.78,P〈0.05）;along with the increasing severity of non-specific digestive disorder,the ATG16L1 gene mRNA expression in colon was gradually increased（P〈0.05）;genotype AA had the lowest mRNA expression in fibre-deficient diet group（P〈0.05）.These results showed that ATG16L1 gene was associated with the genetic susceptibility to NSDD in rabbit.

关 键 词：非特异性消化道紊乱 ATG16L1 多态性 家兔 

分 类 号：S829.1[农业科学—畜牧学]