HPLC法测定甲醛甲酚溶液中甲醛的含量  

Determination of Formaldehyde in Cresol and Formaldehyde Solution by HPLC

作　　者：贾艾玲[1] 王红梅[1] 李玲玲[1] 

机构地区：[1]厦门市药品检验所,厦门361012

出　　处：《中国药品标准》2013年第4期263-265,共3页Drug Standards of China

基　　金：2012年市重大科技项目(项目编号3502Z20121035;厦科联{2012}93号)

摘　　要：目的：建立甲醛甲酚溶液中甲醛含量的HPLC测定方法.方法：以十八烷基硅烷键合硅胶为固定相,以乙腈-磷酸溶液（取磷酸5.75 g,加水至1000 mL,用浓氨水调节pH值至2.85）（50∶50）为流动相,检测波长为336 nm,柱温40 ℃.结果：甲醛在10～100 μg范围内线性关系良好（r=0.9997）,平均回收率为98.6%,RSD为0.8%（n=6）.结论：本方法灵敏、准确,选择性和重现性好,可作为甲醛甲酚溶液中甲醛的质量控制方法.Objective ：To establish an HPLC method for determining the content of formaldehyde in cresol and formaldehyde solution. Methods ： Using a column packed with octadecylsilane bonded silica gel as analytical column. The mobile phase was acetonitrile-phos- phoric acid solution （ dissolve 5.75 g of phosphoric acid in 1000 mL of water, mix well, and adjust pH to 2. 85 with concentrated am- monia solution） （50： 50）. The detection wavelength was 336 nm. The temperature of the column was 40 ℃. Results： There was a good linear relationship within the content of formaldehyde added of 10-1001.Lg（ r=0. 9997 ）. The average recovery was 98. 6% （RSD = 0. 8%, n = 6）. Conclusion ： The method is sensitive, accurate, reliable, and can be applied to quality control of formaldehyde in cresol and formaldehyde solution.

关 键 词：甲醛甲酚溶液 甲醛 高效液相色谱法 含量测定 

分 类 号：R921.2[医药卫生—药学]