高效液相色谱法测定塑料输液容器中四种常用抗氧剂的含量  

Content Determination of Four Common Antioxidants in Plastic Infusion Containers by HPLC

作　　者：陈瑜[1] 王丹丹[1] 金立[1] 

机构地区：[1]浙江省食品药品检验所,杭州310004

出　　处：《中国药品标准》2013年第4期279-281,共3页Drug Standards of China

摘　　要：目的：建立塑料输液容器中四种常用抗氧剂含量测定方法.方法：采用Dikma Diamonsil C18色谱柱（4.60 mm×250 mm,5 μm）,流动相为乙腈-四氢呋喃-水（60∶30∶10）,柱温30 ℃,检测波长275 nm.结果：四种抗氧剂在0～0.2 mg·mL-1范围内均呈良好的线性关系（r=0.999）,抗氧剂1010回收率为97.2%（RSD=3.7%）,抗氧剂1330回收率为95.4%（RSD=3.6%）,抗氧剂1076回收率为94.8%（RSD=3.8%）,抗氧剂168回收率为95.2%（RSD=4.4%）.结论：该方法准确、灵敏、简便,适用于测定塑料输液容器中四种常用抗氧剂的含量.Objective： To develop a method for content determination of four common antioxidants in plastic infusion containers by HPLC. Method： The analysis was performed on Dikma Diamonsil C18 column（4. 0 mm × 250 mm,5 μm）with UV detection at 275 nm. The mobile phase was acetonitrile-THF-water （60： 30： 10） and column temperature was 30 ℃. Results： The method had a linear range between 0 - 0. 2 mg · mL^-1 （ r = 0. 999）. The average recovery of Antioxidant 1010 was 97.2% （ RSD = 3.7% ）. Antioxidant 1330 was 95.4% （ RSD = 3. 6% ）, Antioxidant 1076 was 94. 8% （ RSD = 3. 8% ）, and Antioxidant 168 was 95. 2% （ RSD = 4. 4% ）. Condusion： This method is accurate, sensitive and simple, it can be used for content determination of four common antioxidants in plastic infusion containers.

关 键 词：高效液相色谱法 塑料输液容器 抗氧剂 

分 类 号：R921.2[医药卫生—药学]