稳定同位素法研究川芎哚体内代谢  

Study of urinary metabolites of perlolyrine in rats by stable isotope tracing method

作　　者：唐刚华[1] 姜国辉[2] 王世真[2] 郑连芳[2] 

机构地区：[1]第一军医大学南方医院,广州510515 [2]中国医学科学院-中国协和医科大学北京协和医院,北京100730

出　　处：《核技术》2000年第11期753-756,共4页Nuclear Techniques

摘　　要：分别灌胃给予川芎哚和［２－１５Ｎ］川哚后，尿样经酶水解、碱化、有机溶剂提取。有机相（碱性和中性部分）经浓缩并硅烷化；水相经酸化、吹干提取（提取物为酸性水溶部分）并硅烷化。用ＧＣ－ＭＳ测定。在碱性和中性提取物中，检出原型川穹哚及其一种代谢物；在酸性水溶性提取物中，检出川芎哚的另两种代谢物。结果表明，川芎哚体内代谢途径可能是川芎哚羟基化和川芎哚羟甲基的氧化。After oral administration of perlolyrine and [2-15N] perlolyrine, urines of rats were hydrolyzed with glucuronidase, basified with NaHCO3-Na2CO3, and extracted with ethyl ether-iso-propyl alcohol etc. The organic phases (neutral and basic fractions) were concentrated for TMS derivatives. The aqueous phase were acidified with sulfuric acid, taken to dryness and extracted with methanol (water soluble acidic fractions) and concentrated for TMS derivatives. The TMS derivatives were determined by GC-MS. Perlolyrine and one metabolite were found from the neutral and basic fractions, and two different metabolites were found from the water soluble acidic fractions. It is proposed that the major metabolic pathways of perlolyrine are the hydroxylation of perlolyrine and the oxidition of its hydroxylmethyl group.

关 键 词：川芎哚 代谢 中药 GC-MS 稳定同位素法 

分 类 号：R817.1[医药卫生—影像医学与核医学] R285.5[医药卫生—放射医学]