人血清IgG的^(188)Re标记条件初探  

A Preliminary study on the labelling of immunoglobulin with ^(188)Re

作　　者：丁自更[1] 于俊峰[1] 尹端沚[1] 胡伟清 汪勇先[1] 王根凤[2] 张茹萍[2] 来文[2] 谢弘[2] 

机构地区：[1]中国科学院上海原子核研究所,上海201800 [2]中国科学院上海细胞生物研究所,上海200031

出　　处：《核技术》2000年第11期762-766,共5页Nuclear Techniques

摘　　要：以含葡萄糖酸钠配体的ＳｎＣｌ２为还原液，还原Ｎａ１８８ＲｅＯ４后，标记人血清ＩｇＧ，在ｐＨ＝３的条件下， １ｇ／Ｌ的蛋白质用高于５０００倍摩尔过量的抗坏血酸（ＡＡ）还原２ｈ后，与２．６×１０－２－８．０×１０－２ｍｏｌ／Ｌ的ＳｎＣｌ２－葡萄糖酸钠溶液及１８８－ＲｅＯ４－反应３ｈ，可以得到９０％的标记率，在该标记条件下，对影响标记率的因素作了初步的研究和探讨。SnCl2 reduction solution together with sodium gluconate was chosen to reduce Na188ReO4- and label human IgG. The labelling conditions were optimized. It was found that the labelling efficiency could reach 90% under the following condition: 1g/L protein with 5000 mole ratio AA in 2h reducing time at pH= 3, in mingled with 2.6×10-2- 8.0× 10-2mol/L SnCl2 in sodium gluconate and 188ReO4- at ambient temperature. The factors effecting labelling efficiency were studied and discussed.

关 键 词：标记率 人血清白蛋白 铼^188标记 放射性核素 

分 类 号：R817.8[医药卫生—影像医学与核医学]