新型血栓显像剂～(99m)Tc-重组水蛭素的实验研究  

Experimental studies on thrombus imaging with ^(99m)Tc-recombinant hirudin

作　　者：张荣军[1] 万卫星[1] 周彦 谭成[1] 陶永辉[1] 王博诚[1] 韩玉珉[2] 金坚[1] 

机构地区：[1]核医学国家重点实验室,江苏省原子医学研究所无锡214063 [2]中国科学院生物物理研究所,北京100101

出　　处：《核技术》2000年第11期771-775,共5页Nuclear Techniques

基　　金：卫生部科研基金资助!(96-1-344)

摘　　要：采用直接法和间接法进行了９９ｍＴｃ标记重组水蛭素的研究，结果表明，间接法制备的９９ｍＴｃ－重组水蛙素具有更高的放化纯和稳定性。更适合于血栓模型的显像研究。９９ｍＴｃ－重组水蛙素在小鼠体内的分布表明，心、肝、脾、肺和脑的放射性分布均较低；放射性主要集中在肾脏，１５ｍｉｎ时肾脏放射性可达总注入剂量的６５％；且血液中放射性清除较快。兔血栓模型显像结果表明，９９ｍＴｃ－重组水蛙素可在血栓部位浓聚；注入后１ｈ即可使血栓清晰显影。因此，９９ｍＴｃ－重组水蛙素有望成为一种新型的血栓显像剂。Comparsion of two labeling methods showed that 99mTc-recombinant hirudin (99mTc-rH) prepared by modified 2-imiothiolane method had higher radiochemical purity and stability than that by direct labeling method. Biodistribution of 99mTc-rH in mice showed that the radioactivity in heart, lung, liver and spleen was very low, while that in kidney was high. 99mTc-rH was cleared rapidly in blood and excreted mostly from kidney. The arterial and venous thrombi in rabbits were clearly imaged 60min after injection of 99mTc-rH, T/B ratio was 2.24 and 1.95 respectively. The rH would be a potential agent for diagnosis of thrombotic disease.

关 键 词：水蛭素 血栓 诊断 血栓显像剂 锝^99m标记 

分 类 号：R543.130.4[医药卫生—心血管疾病] R817.42[医药卫生—内科学]