地佐辛对神经病理性疼痛大鼠行为学及脊髓肿瘤坏死因子-α和前列腺素E_2水平的影响  

Influence of dezocine on behavior and levels of tumor necrosis factor-α and prostaglandin E_2 in rats with neuropathic pathologic pain

作　　者：陈莺[1] 朱晨[1] 

机构地区：[1]南方医科大学南方医院麻醉科,广东广州510515

出　　处：《新乡医学院学报》2013年第9期688-691,共4页Journal of Xinxiang Medical University

基　　金：广东省医学科研基金面上项目(编号:A20120366)

摘　　要：目的观察地佐辛对神经病理性疼痛大鼠行为学及脊髓肿瘤坏死因子-α(TNF-α)和前列腺素E2(PGE2)水平的影响。方法成年雄性大鼠30只随机分为假手术组、模型组和地佐辛组。模型组和地佐辛组大鼠进行坐骨神经慢性压迫性损伤模型制备,假手术组大鼠仅暴露坐骨神经,不进行坐骨神经结扎;地佐辛组大鼠于术后即刻至术后7 d,每日腹腔注射地佐辛5 mg.kg-1,假手术组和模型组大鼠给予等体积的生理盐水。检测术前1 d、术后1、3、5、7、14 d 3组大鼠机械性缩足反射阈值(MWT),并于术后14 d测定大鼠脊髓TNF-α及PGE2水平。结果 3组大鼠术前MWT比较差异无统计学意义(P>0.05),假手术组术后1、3、5、7、14 d大鼠MWT与术前比较差异均无统计学意义(P>0.05),模型组和地佐辛组术后1、3、5、7、14 d大鼠MWT显著低于术前(P<0.05);术后1、3、5、7、14 d,模型组和地佐辛组大鼠MWT均显著低于假手术组(P<0.05),地佐辛组大鼠MWT均显著高于模型组(P<0.05)。模型组和地佐辛组大鼠脊髓中TNF-α和PGE2水平显著高于假手术组(P<0.05),地佐辛组大鼠脊髓中TNF-α和PGE2水平显著低于模型组(P<0.05)。结论地佐辛可抑制坐骨神经慢性压迫性损伤大鼠的神经病理性疼痛,其机制可能与其降低大鼠脊髓中TNF-α和PGE2水平有关。Objective To investigate the influence of dezocine on behavior and the levels of tumor necrosis factor-α（TNF-α） and prostaglandin E2 （PGE2 ） in rats with neuropathic pathologic pain. Methods Thirty adult male rats were ran- domly divided into sham-operation group, model group and dezocine group. The rats in model group and dezocine group were made the model of chronic sciatic nerve oppressive injury; the sciatic nerve of rats in sham-operation group were exposed only, and the sciatic nerve were not ligature＆ The rats in dezocine group were treated with dezocine 5 mg · kg^-1 by intraperitoneal injection every day from the beginning to seven days after operation;but the rats in sham-operation group and model group were treated with isochoric normal saline. The changes of mechanical withdrawal threshold（MWT） of rats in the three groups were detected at the time of 1 day before operation and 1 day,3,5,7,14 days after operation;14 days after operation, the levels of TNF-α and PGE2 in spinal cord of rats were detected. Results There was no significant difference in MWT among the three groups before treatment（P 〉 0.05）. In sham-operation group,the MWTs at 1 day ,3,5,7,14 days after operation had no signifi- cant difference compared with those at pre-operation （P 〉 0.05 ） ;but the MWTs in model group and dezocine group at 1 day ,3,5, 7,14 days after operation were significantly lower than those at preoperation（ P 〈 0.05 ）. The MWTs in model group and dezocine group were significantly lower than those in sham-operation group（ P 〈0.05 ） ,and the MWTs in dezocine group were significantly higher than those in model group at 1 day,3,5,7,14 days after operation（ P 〈 0.05 ）. The levels of TNF-α and PGE2 in spinal cord of rats in model group and dezocine group were significantly higher than those in sham-operation group（ P 〈 0.05 ） ;but the levels of TNF-α and PGE2 in spinal cord of rats in dezocine group were significantly higher than those in model group （P 〈 0. 05）

关 键 词：地佐辛 神经病理性疼痛 机械性缩足反射阈值 肿瘤坏死因子-Α 前列腺素E2 

分 类 号：R971[医药卫生—药品]