转基因烟草中过量生长素对叶片生长发育的影响  

EFFECT OF OVER-PRODUCED IAA ON LEAF PHENOTYPE IN iaaM TRANSGENIC TOBACCO(NICOTIANA TABACUM L.)

作　　者：李朝霞[1,2] 殷娴[1,3] 江一希[1] 姜洋[1] 肖尊安[1] 

机构地区：[1]北京师范大学生命科学学院北京市重点实验室,北京100875 [2]北京教学植物园,北京100061 [3]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《北京师范大学学报（自然科学版）》2013年第4期369-373,共5页Journal of Beijing Normal University(Natural Science)

基　　金：国家转基因生物新品种培育科技重大专项基金资助项目(20092X08009-089B)

摘　　要：分别构建了iaaM、iaaM和ipt共表达植物转化载体,农杆菌介导转化烟草,RT-PCR鉴定转基因株系,ELISA方法分析叶片IAA、IPA和ZR含量.结果表明:叶片IAA含量高低依次为株系iaaM10、iaaM_ipt3和野生型;而细胞分裂素含量高低依次为iaaM_ipt3、野生型和iaaM10.随着内源IAA水平升高,气孔密度减小、保卫细胞长度增加、栅栏组织细胞增大和叶片厚度增加.讨论了生长素和细胞分裂素含量对叶片细胞生长发育的影响.The correlation between endogenous IAA level and leaf phenotype was investigated by introducing tryptophan monooxidase gene（iaaM）,or co-expression of iaaM and isopentenyl transferase（ipt） into tobacco（Nicotiana tabacum L.） plants via A.tumefaciens transfection.Semiquantitative RT-PCR,ELISA and microscopy were used,respectively,to analyze transgene expression level,to quantitate endogenous phytohormones（zeatin riboside-ZR,isopentenyl adenosine-IPA,indole-3-acetic acid-IAA）,leaf stoma density,guard cell length,palisade tissue cell size and leaf cross-section.Data showed that in comparison with the wild type plants,transgenic plants-iaaM10 displayed the highest content in IAA but the lowest content in cytokinin;whereas iaaM-ipt3 showed the highest level in cytokinin.With increasing IAA levels,stomatal density decreased,whereas guard cell length,palisade tissue cell length and width,and thickness of leaf cross-section all increased.A possible effect of cytokinin-to-auxin ratio on plant phenotype was discussed.

关 键 词：烟草 色氨酸单氧化酶基因 异戊烯基转移酶基因 生长素 细胞分裂素 

分 类 号：S432.1[农业科学—植物病理学]