3个中籼稻骨干恢复系对稻瘟病和褐飞虱抗性改良效果的评价  

Evaluation on Effect of Resistance Improvement of Rice Blast and Brown Planthopper for Three Dominant Indica Restorer Lines

作　　者：陈凯[1,2] 张强[1,2] 潘晓飚 李美[4] 孟丽君[4] 徐正进[1] 徐建龙[2,4] 黎志康[2,4] 

机构地区：[1]沈阳农业大学/农业部作物生理生态遗传育种重点开放实验室,辽宁沈阳110161 [2]中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程,北京100081 [3]台州市农业科学研究院,浙江临海317000 [4]中国农业科学院深圳生物育种创新研究院,广东深圳518083

出　　处：《核农学报》2013年第8期1069-1080,共12页Journal of Nuclear Agricultural Sciences

基　　金：国家"948"计划(2011-G2B);国家"863"计划(2012AA101101)

摘　　要：利用分子标记辅助抗性基因选择和针对轮回亲本表型严格筛选相结合的方法,将三黄占2号的抗稻瘟病主基因Pi-GD-1(t)、Pi-GD-2(t)、Pi-GD-3(t)和主效QTL GLP8-6(t)(分别简称G1、G2、G3和G8)及IR65482-7-216-1-2-B(简称为IR65482)的抗褐飞虱基因Bph18(t)导入到明恢86、蜀恢527和浙恢7954等3个骨干中籼恢复系,获得带有不同抗瘟基因组合的改良恢复系明恢86-G2、明恢86-G1-G2、蜀恢527-G1-G2、蜀恢527-G2、浙恢7954-G1-G2、浙恢7954-G1-G2-G8和携有抗褐飞虱Bph18(t)基因的蜀恢527-Bph18(t);从3个恢复系与不同抗源杂交F1的复交后代中选育出带有抗稻瘟病和兼抗稻瘟病、稻飞虱的双基因或多基因聚合系浙蜀-G2-G8、明浙-G2-G8、明浙-G1-G2-G8、浙恢7954-G1-G8(t)-Bph18(t)和明浙-G1-G2-Bph18(t)。这12个抗性改良恢复系对20个稻瘟病菌均表现出良好的抗性,蜀恢527-Bph18(t)、浙恢7954-G1-G8(t)-Bph18(t)和明浙-G1-G2-Bph18(t)对褐飞虱的抗性也达到中抗以上水平。多数抗性改良恢复系及其与不育系II-32A的测交种在正常条件下的产量和结实率与原来的恢复系及相应杂交种相仿或优于原来的恢复系及相应杂交种,具有生产应用价值。研究表明,抗稻瘟病基因在不同恢复系背景下的抗性表达完全,对恢复系稻瘟病改良的效果明显,而抗性基因Bph18(t)对褐飞虱的改良效果与遗传背景有关。对分子标记回交和复交改良恢复系的抗病虫性进行了讨论。The novel blast resistance genes,Pi-GD-1（t）,Pi-GD-2（t）,Pi-GD-3（t） and GLP8-6（t） from rice variety Sanhuangzhan2（here abbreviated them as G1,G2,G3 and G8,respectively） and brown planthopper（BPH） resistance gene,Bph18（t） from IR65482-7-216-1-2-B（abbreviated as IR65482） were introgressed into three popular rice restorer lines,Minghui86,Shuhui527 and Zhehui7954 for wild abortive cytoplasmic male sterility by marker-assisted backcross combined with stringent phenotypic selections against recurrent parents.The eleven derived blast resistance restorer lines,Minghui86-G2,Minghui86-G1-G2,Shuhui527-G2,Shuhui527-G1-G2,Zhehui7954-G1-G2,Zhehui7954-G1-G2-G8,Zheshu-G2-G8,Zhehui7954-G1-G8（t）-Bph18（t）,Mingzhe-G2-G8,Mingzhe-G1-G2-G8 and Mingzhe-G1-G2-Bph18（t）,demonstrated similar or wider blast resistance spectrum as compared with the donor parent,Sanhuangzhan 2.The three derived BPH resistant restorer lines,Shuhui527-Bph18（t）,Zhehui7954-G1-G8（t）-Bph18（t） and Mingzhe-G1-G2-Bph18（t） showed resistance or moderate resistance to BPH.The newly developed blast and BPH resistant restorers and their derived hybrids with the sterile line II-32A were identical or superior to their respective original versions for agronomic traits under disease free condition,suggesting these resistance improved restorer lines could be use in hybrid rice production.The results indicated that the blast genes could completely express their dominant resistance in different restorer line backgrounds,suggesting their immense breeding value in blast resistance improvement for hybrid rice.However,effect of resistant improvement of Bph18（t） for BPH depends on genetic background.Improvement of resistance of blast and BPH for restorer lines by molecular marker-assisted backcross and pyramiding was deeply discussed.

关 键 词：水稻恢复系 稻瘟病 褐飞虱 抗性改良 标记辅助选择 

分 类 号：S511.21[农业科学—作物学]