强光下H_2S对石斛DNA甲基化及抗氧化系统的影响  

Effects of Sodium Hydrosulfide on DNA Methylation and Antioxidant System of Dendrobium officinale under High Light Stress

作　　者：关蕾[1] 樊洪泓[1] 陈沙沙[1] 林毅[1] 蔡永萍[1] 金青[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《核农学报》2013年第8期1090-1098,共9页Journal of Nuclear Agricultural Sciences

基　　金：安徽省自然科学基金青年基金(10040606Q22);安徽省高等学校优秀青年人才基金(2011SQRL053);校稳定人才基金项目(yj2008-27);校长青年基金项目;安徽省中药材产业体系;安徽省教育厅重点自然科学项目(KJ2011A109)

摘　　要：本试验以铁皮石斛为材料,研究强光胁迫下外源硫化氢对铁皮石斛的抗氧化系统及基因组DNA的甲基化水平和模式变化的影响。结果表明:200μmol.L-1的硫氢化钠(NaHS,H2S的供体)可显著促进SOD、CAT和POD的酶活性、降低MDA含量,缓解了强光胁迫对铁皮石斛造成的氧化损伤。而高浓度NaHS(600μmol.L-1)处理则加重了石斛的氧化损伤。甲基化敏感扩增多态性(Methylation sensitive amplified polymorphism,MSAP)分析表明,强光胁迫下0、200和600μmol.L-1NaHS处理(T1、T3、T5)的石斛DNA甲基化比率分别为29.6%、33%和28.9%,均低于对照(正常光照,35.4%)。强光下T1与对照CK相比、T3和T5与T1相比,石斛基因组DNA的去甲基化和甲基化变化的位点比例分别为6.4%、7.7%、6.7%和9.0%、8.1%、9.2%。通过对差异条带的回收测序和Blast比对,发现其中有与光合作用有关的合成酶、与抗逆相关的转录因子等同源的片段。The effects of sodium hydrosulfide（NaHS,H2S donor） on the antioxidant system and the DNA methylation profile patterns of Dendrobium officinale under high light stress were studied in this paper.The results showed that 200μmol·L-1NaHS could significantly promote the enzyme activity of SOD,CAT and POD,reduce the content of MDA and relieve oxidative damage caused by high light stress on Dendrobium officinale.While high concentration NaHS（600μmol·L-1） increased the oxidative damage.The analysis of methylation sensitive amplified polymorphism（MSAP） showed that the level of DNA methylation of control group was highest（35.4%）.Then the seedling were treated with 0,200,600μmol·L-1NaHS,the level of DNA methylation was 29.6%,33% and 28.9%,respectively.With different concentration of NaHS（0,200 and 600μmol·L-1）,demethylation and methylation of DNA were 6.4%,7.7%,6.7% and 9.0%,8.1%,9.2%,respectively.Several variable MSAP profiles were isolated and sequenced.By Blast analysis,the relevant photosynthetic enzymes and transcription factor were found.

关 键 词：铁皮石斛 硫化氢 DNA甲基化 甲基化敏感扩增多态性 

分 类 号：S567.239[农业科学—中草药栽培]