转基因水稻纯系对褐飞虱的抗性研究(英文)  

Studies on Resistance to Brown Planthopper in Transgenic Rice Pure Lines

作　　者：唐克轩[1] 孙小芬[1] 姚剑虹[1] 戚华雄[2] 卢兴桂 

机构地区：[1]复旦大学生命科学院 [2]湖北农科院杂交水稻研究中心

出　　处：《复旦学报（自然科学版）》2000年第4期436-440,共5页Journal of Fudan University：Natural Science

基　　金：SupportedbytheRockefellerFoundation USA(Grantno .RF970 0 1# 5 94) ;SupportedbyChinaNationalHigh Tech"86 3"program(Grantno .1

摘　　要：利用基因枪法将含潮霉素抗性基因、GUS报告基因和雪花莲凝集素基因的 2个质粒 pWRG15 15和pRSSGNA1共同转化粳稻品种鄂宜 10 5的成熟胚诱导的愈伤组织 .从轰击的 15 2块愈伤组织中共再生出 2 6株独立转基因植株 .PCR/Southern印迹法分析发现 ,73%的转基因植株含有所有 3个外源基因 .遗传分析证实外源基因在转基因植株后代中以孟德尔方式遗传 .从其R1代亲本为孟德尔 3∶1方式遗传的R2代中 ,鉴定出 3个表达所有 3个外源基因的独立转基因植株纯系 .抗虫鉴定结果发现这些纯系对褐飞虱具有明显的抑制作用 .Mature seed derived callus from japonica rice cv. Eyi 105 was co transformed with two plasmids,pWRG1515 and pRSSGNA1,containing the selectable marker hygromycin phosphotransferase gene ( hpt ),the reporter β glucuronidase gene ( gus A) and the snowdrop ( Galanthus nivalis ) lectin gene ( gna ) via particle bombardment.Twenty six independent transgenic rice plants were regenerated from 152 bombarded callus. Seventy three percent of transgenic plants contained all the three genes, revealed by PCR/Southen blot analysis.Genetic analysis confirmed Mendelian segregation of transgenes in progeny. From R2 generations with their R1 parent plants showing 3∶1 Mendelian segregation patterns, three independent homozyous lines expressing all the three transgenes were identified. Insect bioassay results showed that these homozygous lines had significant inhibition to brown planthopper.

关 键 词：水稻 共转化 转基因植株 褐飞虱 

分 类 号：S511.101[农业科学—作物学]