正交试验优选土茯苓总黄酮的最佳提取工艺  

The Optimum Technique for Extracting Total Flavonoids from Smilax glabra Roxb. by Orthogonal Test

作　　者：刘嘉琪[1] 杨晶[1] 王宝昌[1] 付晓雪[1] 史丽颖[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622

出　　处：《安徽农业科学》2013年第17期7468-7470,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选土茯苓总黄酮的最佳提取工艺。[方法]在单因素试验的基础上,以土茯苓总黄酮的收率为指标,采用正交试验法优选土茯苓总黄酮的最佳提取工艺条件。[结果]土茯苓总黄酮的最佳提取工艺为:料液比为1∶20,乙醇浓度为60%,提取温度为95℃,回流提取2次,每次2 h;在此条件下,总黄酮的收率为8.34%。[结论]该工艺操作简单、稳定、可行。Abstract [ Objective ] To optimize the extracting technology of total flavonoids from Smilax glabra Roxb.. [ Method ] On the basis of single factor test, using the yield of total flavonoids as indicator, the optimum extracting technology of total flavonoids was obtained by orthogonal test. [ Result ] The optimum extracting technology was as follows：solid-liquid ratio 1：20, 60% ethanol, extracting 2 times at 95 ~C for 2 hours. The yield of total flavonoids was 8.34% under the above conditions. [ Conclusion] The extracting technology is simple, stable and feasible.

关 键 词：土茯苓（RHIZOMA SMILACIS GLABRAE） 总黄酮 提取工艺 正交试验 

分 类 号：S567[农业科学—中草药栽培]