饲料添加剂吖啶黄与M相互作用的光谱研究  

Study on the Fluorescence Quenching Mechanism between M and Acriflavine

作　　者：徐科[1] 徐红[1] 刘红[1] 黄亚励[1] 

机构地区：[1]贵阳医学院,贵州贵阳550004

出　　处：《安徽农业科学》2013年第17期7558-7560,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅社会攻关项目(黔科合SY字[2011]3128)

摘　　要：研究了在pH=1.98、T=20℃时,采用荧光和同步荧光研究了吖啶黄(Acr)与M的相互作用。根据Stem-Volmer方程计算出吖啶黄与M的配位作用的结合常数、猝灭常数及结合位数,探索了吖啶黄与M配位机理。结果表明,吖啶黄与Ag+和Mg2+配位能力较强,其配位作用导致吖啶黄荧光猝灭,而Fe3+与吖啶黄配位作用较弱,主要通过分子间的碰撞导致吖啶黄荧光猝灭。The characteristics of M （Ag ＋ , Mg , ） and Aefiflavine were studied by fluereseence and synchronous fluerescence spectrosco- py in aqueous solution according to Stem-Volmer equation. The results showed that Ag ＋ and Mg2 ＋ ions had a powerful ability to quench the Acri- flavine fIuerescenee via a nor, radlative energy transfer mec^mism in the pH = 1.98 B. R buffer system and T = 20 ℃. But Fe3 ＋ had quenched the Acriflavine fluerescenee via a Molecular collisions transfer mechanism. The quenching rate constants, the coordination constants and the coordi- nation sites of the static quenching were calculated.

关 键 词：吖啶黄 配位 分子碰撞 荧光猝灭 

分 类 号：S131[农业科学—农业基础科学]